In vivo monitoring of rat brain metabolites during vigabatrin treatment using localized 2D-COSY.
A two-dimensional COSY-based localization sequence was designed to allow the in vivo monitoring of proton metabolites in rat brain [particularly gamma-aminobutyric acid (GABA), glutamine, taurine and myo-inositol]. The sequence incorporated OSIRIS signal localization, B1-insensitive water suppressio...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2003
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| _version_ | 1797071013738971136 |
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| author | Welch, J Bhakoo, K Dixon, R Styles, P Sibson, N Blamire, A |
| author_facet | Welch, J Bhakoo, K Dixon, R Styles, P Sibson, N Blamire, A |
| author_sort | Welch, J |
| collection | OXFORD |
| description | A two-dimensional COSY-based localization sequence was designed to allow the in vivo monitoring of proton metabolites in rat brain [particularly gamma-aminobutyric acid (GABA), glutamine, taurine and myo-inositol]. The sequence incorporated OSIRIS signal localization, B1-insensitive water suppression and phase-sensitive COSY acquisition. The method was used to study the effects of the GABA-transaminase inhibitor vigabatrin on rat brain metabolite concentrations. Wistar rats were treated daily for 3 days with an oral dose of vigabatrin (200 mg/kg, n = 4). Localized COSY spectra were obtained during a 120 min acquisition from a 270 microl central brain voxel and compared with nine untreated control animals. Significant elevations were observed in GABA (267% of control, p < 0.005, Mann-Witney test), glutamine (130% of control, p < 0.005) and taurine (113% of control, p < 0.05). Changes in GABA and taurine were consistent with previous data on the action of Vigabatrin, and support a previously hypothesized link between these compounds. The increase in glutamine was more surprising and may reflect the balance between the level and/or site of GABA-transaminase inhibition and downregulation of GABA synthesis. |
| first_indexed | 2024-03-06T22:47:09Z |
| format | Journal article |
| id | oxford-uuid:5d8e8d96-cf17-4b11-8f74-553f8bb204e0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:47:09Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:5d8e8d96-cf17-4b11-8f74-553f8bb204e02022-03-26T17:35:12ZIn vivo monitoring of rat brain metabolites during vigabatrin treatment using localized 2D-COSY.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5d8e8d96-cf17-4b11-8f74-553f8bb204e0EnglishSymplectic Elements at Oxford2003Welch, JBhakoo, KDixon, RStyles, PSibson, NBlamire, AA two-dimensional COSY-based localization sequence was designed to allow the in vivo monitoring of proton metabolites in rat brain [particularly gamma-aminobutyric acid (GABA), glutamine, taurine and myo-inositol]. The sequence incorporated OSIRIS signal localization, B1-insensitive water suppression and phase-sensitive COSY acquisition. The method was used to study the effects of the GABA-transaminase inhibitor vigabatrin on rat brain metabolite concentrations. Wistar rats were treated daily for 3 days with an oral dose of vigabatrin (200 mg/kg, n = 4). Localized COSY spectra were obtained during a 120 min acquisition from a 270 microl central brain voxel and compared with nine untreated control animals. Significant elevations were observed in GABA (267% of control, p < 0.005, Mann-Witney test), glutamine (130% of control, p < 0.005) and taurine (113% of control, p < 0.05). Changes in GABA and taurine were consistent with previous data on the action of Vigabatrin, and support a previously hypothesized link between these compounds. The increase in glutamine was more surprising and may reflect the balance between the level and/or site of GABA-transaminase inhibition and downregulation of GABA synthesis. |
| spellingShingle | Welch, J Bhakoo, K Dixon, R Styles, P Sibson, N Blamire, A In vivo monitoring of rat brain metabolites during vigabatrin treatment using localized 2D-COSY. |
| title | In vivo monitoring of rat brain metabolites during vigabatrin treatment using localized 2D-COSY. |
| title_full | In vivo monitoring of rat brain metabolites during vigabatrin treatment using localized 2D-COSY. |
| title_fullStr | In vivo monitoring of rat brain metabolites during vigabatrin treatment using localized 2D-COSY. |
| title_full_unstemmed | In vivo monitoring of rat brain metabolites during vigabatrin treatment using localized 2D-COSY. |
| title_short | In vivo monitoring of rat brain metabolites during vigabatrin treatment using localized 2D-COSY. |
| title_sort | in vivo monitoring of rat brain metabolites during vigabatrin treatment using localized 2d cosy |
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