Modified rare earth semiconductor oxide as a new nucleotide probe.
Recent rapid developments in biological analysis, medical diagnosis, pharmaceutical industry, and environmental control fuel the urgent need for recognition of particular DNA sequences from samples. Currently, DNA detection techniques use radiochemical, enzymatic, fluorescent, or electrochemilumines...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2006
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| _version_ | 1797071063482368000 |
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| author | Shrestha, S Mills, C Lewington, J Tsang, S |
| author_facet | Shrestha, S Mills, C Lewington, J Tsang, S |
| author_sort | Shrestha, S |
| collection | OXFORD |
| description | Recent rapid developments in biological analysis, medical diagnosis, pharmaceutical industry, and environmental control fuel the urgent need for recognition of particular DNA sequences from samples. Currently, DNA detection techniques use radiochemical, enzymatic, fluorescent, or electrochemiluminescent methods; however, these techniques require costly labeled DNA and highly skilled and cumbersome procedure, which prohibit any in-situ monitoring. Here, we report that hybridization of surface-immobilized single-stranded oligonucleotide on praseodymium oxide (evaluated as a biosensor surface for the first time) with complimentary strands in solution provokes a significant shift of electrical impedance curve. This shift is attributed to a change in electrical characteristics through modification of surface charge of the underlying modified praseodymium oxide upon hybridization with the complementary oligonucelotide strand. On the other hand, using a noncomplementary single strand in solution does not create an equivalent change in the impedance value. This result clearly suggests that a new and simple electrochemical technique based on the change in electrical properties of the modified praseodymium oxide semiconductor surface upon recognition and transduction of a biological event without using labeled species is revealed. |
| first_indexed | 2024-03-06T22:47:49Z |
| format | Journal article |
| id | oxford-uuid:5dca1896-bd75-4639-b6f4-ea098c55adad |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:47:49Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:5dca1896-bd75-4639-b6f4-ea098c55adad2022-03-26T17:36:29ZModified rare earth semiconductor oxide as a new nucleotide probe.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5dca1896-bd75-4639-b6f4-ea098c55adadEnglishSymplectic Elements at Oxford2006Shrestha, SMills, CLewington, JTsang, SRecent rapid developments in biological analysis, medical diagnosis, pharmaceutical industry, and environmental control fuel the urgent need for recognition of particular DNA sequences from samples. Currently, DNA detection techniques use radiochemical, enzymatic, fluorescent, or electrochemiluminescent methods; however, these techniques require costly labeled DNA and highly skilled and cumbersome procedure, which prohibit any in-situ monitoring. Here, we report that hybridization of surface-immobilized single-stranded oligonucleotide on praseodymium oxide (evaluated as a biosensor surface for the first time) with complimentary strands in solution provokes a significant shift of electrical impedance curve. This shift is attributed to a change in electrical characteristics through modification of surface charge of the underlying modified praseodymium oxide upon hybridization with the complementary oligonucelotide strand. On the other hand, using a noncomplementary single strand in solution does not create an equivalent change in the impedance value. This result clearly suggests that a new and simple electrochemical technique based on the change in electrical properties of the modified praseodymium oxide semiconductor surface upon recognition and transduction of a biological event without using labeled species is revealed. |
| spellingShingle | Shrestha, S Mills, C Lewington, J Tsang, S Modified rare earth semiconductor oxide as a new nucleotide probe. |
| title | Modified rare earth semiconductor oxide as a new nucleotide probe. |
| title_full | Modified rare earth semiconductor oxide as a new nucleotide probe. |
| title_fullStr | Modified rare earth semiconductor oxide as a new nucleotide probe. |
| title_full_unstemmed | Modified rare earth semiconductor oxide as a new nucleotide probe. |
| title_short | Modified rare earth semiconductor oxide as a new nucleotide probe. |
| title_sort | modified rare earth semiconductor oxide as a new nucleotide probe |
| work_keys_str_mv | AT shresthas modifiedrareearthsemiconductoroxideasanewnucleotideprobe AT millsc modifiedrareearthsemiconductoroxideasanewnucleotideprobe AT lewingtonj modifiedrareearthsemiconductoroxideasanewnucleotideprobe AT tsangs modifiedrareearthsemiconductoroxideasanewnucleotideprobe |