Evidence that a ryanodine receptor triggers signal transduction in the osteoclast.
We have investigated the effect of the alkaloid ryanodine on the release of intracellularly stored Ca2+ in response to activation of the osteoclast Ca2+ receptor by the surrogate agonist, Ni2+, Ni2+ (6 mM) in the presence of ethylene-glycol bis-(aminoethyl ether) tetraacetic acid (EGTA) (1.2 mM) and...
| Những tác giả chính: | , , , , , |
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| Định dạng: | Journal article |
| Ngôn ngữ: | English |
| Được phát hành: |
1992
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| _version_ | 1826274825489874944 |
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| author | Zaidi, M Shankar, V Towhidul Alam, A Moonga, B Pazianas, M Huang, C |
| author_facet | Zaidi, M Shankar, V Towhidul Alam, A Moonga, B Pazianas, M Huang, C |
| author_sort | Zaidi, M |
| collection | OXFORD |
| description | We have investigated the effect of the alkaloid ryanodine on the release of intracellularly stored Ca2+ in response to activation of the osteoclast Ca2+ receptor by the surrogate agonist, Ni2+, Ni2+ (6 mM) in the presence of ethylene-glycol bis-(aminoethyl ether) tetraacetic acid (EGTA) (1.2 mM) and valinomycin (5 microM) induced a transient elevation of cytosolic [Ca2+] in fura 2-loaded osteoclasts. This transient was superimposed upon a small steady elevation of cytosolic [Ca2+] induced by the initial application of valinomycin alone. Ryanodine (10 microM) completely abolished such responsiveness. However, cytosolic [Ca2+] transients were restored when osteoclasts were depolarized by the extracellular inclusion of 100 mM-[K+] in the same solution. Thus, we demonstrate a sensitivity of the osteoclast signal transduction system to ryanodine for the first time to our knowledge. |
| first_indexed | 2024-03-06T22:49:19Z |
| format | Journal article |
| id | oxford-uuid:5e462899-1e32-4e88-bbba-f904597bed2d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:49:19Z |
| publishDate | 1992 |
| record_format | dspace |
| spelling | oxford-uuid:5e462899-1e32-4e88-bbba-f904597bed2d2022-03-26T17:39:33ZEvidence that a ryanodine receptor triggers signal transduction in the osteoclast.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5e462899-1e32-4e88-bbba-f904597bed2dEnglishSymplectic Elements at Oxford1992Zaidi, MShankar, VTowhidul Alam, AMoonga, BPazianas, MHuang, CWe have investigated the effect of the alkaloid ryanodine on the release of intracellularly stored Ca2+ in response to activation of the osteoclast Ca2+ receptor by the surrogate agonist, Ni2+, Ni2+ (6 mM) in the presence of ethylene-glycol bis-(aminoethyl ether) tetraacetic acid (EGTA) (1.2 mM) and valinomycin (5 microM) induced a transient elevation of cytosolic [Ca2+] in fura 2-loaded osteoclasts. This transient was superimposed upon a small steady elevation of cytosolic [Ca2+] induced by the initial application of valinomycin alone. Ryanodine (10 microM) completely abolished such responsiveness. However, cytosolic [Ca2+] transients were restored when osteoclasts were depolarized by the extracellular inclusion of 100 mM-[K+] in the same solution. Thus, we demonstrate a sensitivity of the osteoclast signal transduction system to ryanodine for the first time to our knowledge. |
| spellingShingle | Zaidi, M Shankar, V Towhidul Alam, A Moonga, B Pazianas, M Huang, C Evidence that a ryanodine receptor triggers signal transduction in the osteoclast. |
| title | Evidence that a ryanodine receptor triggers signal transduction in the osteoclast. |
| title_full | Evidence that a ryanodine receptor triggers signal transduction in the osteoclast. |
| title_fullStr | Evidence that a ryanodine receptor triggers signal transduction in the osteoclast. |
| title_full_unstemmed | Evidence that a ryanodine receptor triggers signal transduction in the osteoclast. |
| title_short | Evidence that a ryanodine receptor triggers signal transduction in the osteoclast. |
| title_sort | evidence that a ryanodine receptor triggers signal transduction in the osteoclast |
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