Gene silencing pathway RNA-dependent RNA polymerase of Neurospora crassa: yeast expression and crystallization of selenomethionated QDE-1 protein.

The RNA-dependent RNA polymerase, QDE-1, is a component of the RNA silencing pathway in Neurospora crassa. The enzymatically active carboxy-terminal fragment QDE-1 DeltaN has been expressed in Saccharomyces cerevisiae in the presence and absence of selenomethionine (SeMet). The level of SeMet incorp...

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Hlavní autoři: Laurila, MR, Salgado, P, Makeyev, E, Nettelship, J, Stuart, D, Grimes, J, Bamford, D
Médium: Journal article
Jazyk:English
Vydáno: 2005