An extra dimension in nucleic acid sequence recognition.
Watson-Crick base pairing is a natural molecular recognition process that has been exploited in molecular biology and universally adopted in many fields. An additional mode of nucleic acid sequence recognition that could be used in combination with normal base pairing would add an exta dimension to...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2005
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| _version_ | 1826274900995735552 |
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| author | Fox, K Brown, T |
| author_facet | Fox, K Brown, T |
| author_sort | Fox, K |
| collection | OXFORD |
| description | Watson-Crick base pairing is a natural molecular recognition process that has been exploited in molecular biology and universally adopted in many fields. An additional mode of nucleic acid sequence recognition that could be used in combination with normal base pairing would add an exta dimension to nucleic acid interactions and open up many new applications. In principle the triplex approach could provide this if developed to recognize any DNA sequence. To this end modified nucleosides have been incorporated into triple-helix-forming oligonucleotides (TFOs) and used to recognize mixed sequence DNA with high selectivity and affinity at neutral pH. Continuing developments are directed towards improving TFO affinity at high pH and increasing triplex association kinetics. A number of applications of triplexes are currently being explored. |
| first_indexed | 2024-03-06T22:50:32Z |
| format | Journal article |
| id | oxford-uuid:5ea0b856-a2fb-4385-a970-5113f3859d35 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:50:32Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:5ea0b856-a2fb-4385-a970-5113f3859d352022-03-26T17:41:52ZAn extra dimension in nucleic acid sequence recognition.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5ea0b856-a2fb-4385-a970-5113f3859d35EnglishSymplectic Elements at Oxford2005Fox, KBrown, TWatson-Crick base pairing is a natural molecular recognition process that has been exploited in molecular biology and universally adopted in many fields. An additional mode of nucleic acid sequence recognition that could be used in combination with normal base pairing would add an exta dimension to nucleic acid interactions and open up many new applications. In principle the triplex approach could provide this if developed to recognize any DNA sequence. To this end modified nucleosides have been incorporated into triple-helix-forming oligonucleotides (TFOs) and used to recognize mixed sequence DNA with high selectivity and affinity at neutral pH. Continuing developments are directed towards improving TFO affinity at high pH and increasing triplex association kinetics. A number of applications of triplexes are currently being explored. |
| spellingShingle | Fox, K Brown, T An extra dimension in nucleic acid sequence recognition. |
| title | An extra dimension in nucleic acid sequence recognition. |
| title_full | An extra dimension in nucleic acid sequence recognition. |
| title_fullStr | An extra dimension in nucleic acid sequence recognition. |
| title_full_unstemmed | An extra dimension in nucleic acid sequence recognition. |
| title_short | An extra dimension in nucleic acid sequence recognition. |
| title_sort | extra dimension in nucleic acid sequence recognition |
| work_keys_str_mv | AT foxk anextradimensioninnucleicacidsequencerecognition AT brownt anextradimensioninnucleicacidsequencerecognition AT foxk extradimensioninnucleicacidsequencerecognition AT brownt extradimensioninnucleicacidsequencerecognition |