Sialylation of campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice
<p style="text-align:justify;"> <b>Background:</b> Guillain-Barré syndrome (GBS) is a post-infectious polyradiculoneuropathy, frequently associated with antecedent Campylobacter jejuni (C. jejuni) infection. The presence of sialic acid on C. jejuni lipo-oligosaccharide (...
| Հիմնական հեղինակներ: | , , , , , , , , , , |
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| Ձևաչափ: | Journal article |
| Լեզու: | English |
| Հրապարակվել է: |
Public Library of Science
2012
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| _version_ | 1826274902553919488 |
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| author | Huizinga, R Easton, A Donachie, A Guthrie, J Van Rijs, W Heikema, A Boon, L Samsom, J Jacobs, B Willison, H Goodyear, C |
| author_facet | Huizinga, R Easton, A Donachie, A Guthrie, J Van Rijs, W Heikema, A Boon, L Samsom, J Jacobs, B Willison, H Goodyear, C |
| author_sort | Huizinga, R |
| collection | OXFORD |
| description | <p style="text-align:justify;"> <b>Background:</b> Guillain-Barré syndrome (GBS) is a post-infectious polyradiculoneuropathy, frequently associated with antecedent Campylobacter jejuni (C. jejuni) infection. The presence of sialic acid on C. jejuni lipo-oligosaccharide (LOS) is considered a risk factor for development of GBS as it crucially determines the structural homology between LOS and gangliosides, explaining the induction of cross-reactive neurotoxic antibodies. Sialylated C. jejuni are recognised by TLR4 and sialoadhesin; however, the functional implications of these interactions in vivo are unknown.<br/><br/> <b>Methodology/Principal Findings:</b> In this study we investigated the effects of bacterial sialylation on phagocytosis and cytokine secretion by mouse myeloid cells in vitro and in vivo. Using fluorescently labelled GM1a/GD1a ganglioside-mimicking C. jejuni strains and corresponding (Cst-II-mutant) control strains lacking sialic acid, we show that sialylated C. jejuni was more efficiently phagocytosed in vitro by BM-MΦ, but not by BM-DC. In addition, LOS sialylation increased the production of IL-10, IL-6 and IFN-β by both BM-MΦ and BM-DC. Subsequent in vivo experiments revealed that sialylation augmented the deposition of fluorescent bacteria in splenic DC, but not macrophages. In addition, sialylation significantly amplified the production of type I interferons, which was independent of pDC.<br/><br/> <b>Conclusions/Significance:</b> These results identify novel immune stimulatory effects of C. jejuni sialylation, which may be important in inducing cross-reactive humoral responses that cause GBS. </p> |
| first_indexed | 2024-03-06T22:50:33Z |
| format | Journal article |
| id | oxford-uuid:5ea2cd6d-efe8-4fe1-90a2-ff759255398d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:50:33Z |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:5ea2cd6d-efe8-4fe1-90a2-ff759255398d2022-03-26T17:41:56ZSialylation of campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in miceJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5ea2cd6d-efe8-4fe1-90a2-ff759255398dEnglishSymplectic Elements at OxfordPublic Library of Science2012Huizinga, REaston, ADonachie, AGuthrie, JVan Rijs, WHeikema, ABoon, LSamsom, JJacobs, BWillison, HGoodyear, C <p style="text-align:justify;"> <b>Background:</b> Guillain-Barré syndrome (GBS) is a post-infectious polyradiculoneuropathy, frequently associated with antecedent Campylobacter jejuni (C. jejuni) infection. The presence of sialic acid on C. jejuni lipo-oligosaccharide (LOS) is considered a risk factor for development of GBS as it crucially determines the structural homology between LOS and gangliosides, explaining the induction of cross-reactive neurotoxic antibodies. Sialylated C. jejuni are recognised by TLR4 and sialoadhesin; however, the functional implications of these interactions in vivo are unknown.<br/><br/> <b>Methodology/Principal Findings:</b> In this study we investigated the effects of bacterial sialylation on phagocytosis and cytokine secretion by mouse myeloid cells in vitro and in vivo. Using fluorescently labelled GM1a/GD1a ganglioside-mimicking C. jejuni strains and corresponding (Cst-II-mutant) control strains lacking sialic acid, we show that sialylated C. jejuni was more efficiently phagocytosed in vitro by BM-MΦ, but not by BM-DC. In addition, LOS sialylation increased the production of IL-10, IL-6 and IFN-β by both BM-MΦ and BM-DC. Subsequent in vivo experiments revealed that sialylation augmented the deposition of fluorescent bacteria in splenic DC, but not macrophages. In addition, sialylation significantly amplified the production of type I interferons, which was independent of pDC.<br/><br/> <b>Conclusions/Significance:</b> These results identify novel immune stimulatory effects of C. jejuni sialylation, which may be important in inducing cross-reactive humoral responses that cause GBS. </p> |
| spellingShingle | Huizinga, R Easton, A Donachie, A Guthrie, J Van Rijs, W Heikema, A Boon, L Samsom, J Jacobs, B Willison, H Goodyear, C Sialylation of campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice |
| title | Sialylation of campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice |
| title_full | Sialylation of campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice |
| title_fullStr | Sialylation of campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice |
| title_full_unstemmed | Sialylation of campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice |
| title_short | Sialylation of campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice |
| title_sort | sialylation of campylobacter jejuni lipo oligosaccharides impact on phagocytosis and cytokine production in mice |
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