Probing genuine strong interactions and post-translational modifications in the heterogeneous yeast exosome protein complex.
The characterization of heterogeneous multicomponent protein complexes, which goes beyond identification of protein subunits, is a challenging task. Here we describe and apply a comprehensive method that combines a mild affinity purification procedure with a multiplexed mass spectrometry approach fo...
Автори: | Synowsky, SA, van den Heuvel, R, Mohammed, S, Pijnappel, P, Heck, A |
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Формат: | Journal article |
Мова: | English |
Опубліковано: |
2006
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