A homeostatic clock sets daughter centriole size in flies
Centrioles are highly structured organelles whose size is remarkably consistent within any given cell type. New centrioles are born when Polo-like kinase 4 (Plk4) recruits Ana2/STIL and Sas-6 to the side of an existing “mother” centriole. These two proteins then assemble into a cartwheel, which grow...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Published: |
Rockefeller University Press
2018
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| _version_ | 1826274967722917888 |
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| author | Aydogan, M Wainman, A Saurya, S Steinacker, T Caballe, A Novak, Z Baumbach, J Muschalik, N Raff, J |
| author_facet | Aydogan, M Wainman, A Saurya, S Steinacker, T Caballe, A Novak, Z Baumbach, J Muschalik, N Raff, J |
| author_sort | Aydogan, M |
| collection | OXFORD |
| description | Centrioles are highly structured organelles whose size is remarkably consistent within any given cell type. New centrioles are born when Polo-like kinase 4 (Plk4) recruits Ana2/STIL and Sas-6 to the side of an existing “mother” centriole. These two proteins then assemble into a cartwheel, which grows outwards to form the structural core of a new daughter. Here, we show that in early Drosophila melanogaster embryos, daughter centrioles grow at a linear rate during early S-phase and abruptly stop growing when they reach their correct size in mid- to late S-phase. Unexpectedly, the cartwheel grows from its proximal end, and Plk4 determines both the rate and period of centriole growth: the more active the centriolar Plk4, the faster centrioles grow, but the faster centriolar Plk4 is inactivated and growth ceases. Thus, Plk4 functions as a homeostatic clock, establishing an inverse relationship between growth rate and period to ensure that daughter centrioles grow to the correct size. |
| first_indexed | 2024-03-06T22:51:32Z |
| format | Journal article |
| id | oxford-uuid:5ef07835-18e5-44af-a21c-f829e4a6f7a3 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T22:51:32Z |
| publishDate | 2018 |
| publisher | Rockefeller University Press |
| record_format | dspace |
| spelling | oxford-uuid:5ef07835-18e5-44af-a21c-f829e4a6f7a32022-03-26T17:43:56ZA homeostatic clock sets daughter centriole size in fliesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5ef07835-18e5-44af-a21c-f829e4a6f7a3Symplectic Elements at OxfordRockefeller University Press2018Aydogan, MWainman, ASaurya, SSteinacker, TCaballe, ANovak, ZBaumbach, JMuschalik, NRaff, JCentrioles are highly structured organelles whose size is remarkably consistent within any given cell type. New centrioles are born when Polo-like kinase 4 (Plk4) recruits Ana2/STIL and Sas-6 to the side of an existing “mother” centriole. These two proteins then assemble into a cartwheel, which grows outwards to form the structural core of a new daughter. Here, we show that in early Drosophila melanogaster embryos, daughter centrioles grow at a linear rate during early S-phase and abruptly stop growing when they reach their correct size in mid- to late S-phase. Unexpectedly, the cartwheel grows from its proximal end, and Plk4 determines both the rate and period of centriole growth: the more active the centriolar Plk4, the faster centrioles grow, but the faster centriolar Plk4 is inactivated and growth ceases. Thus, Plk4 functions as a homeostatic clock, establishing an inverse relationship between growth rate and period to ensure that daughter centrioles grow to the correct size. |
| spellingShingle | Aydogan, M Wainman, A Saurya, S Steinacker, T Caballe, A Novak, Z Baumbach, J Muschalik, N Raff, J A homeostatic clock sets daughter centriole size in flies |
| title | A homeostatic clock sets daughter centriole size in flies |
| title_full | A homeostatic clock sets daughter centriole size in flies |
| title_fullStr | A homeostatic clock sets daughter centriole size in flies |
| title_full_unstemmed | A homeostatic clock sets daughter centriole size in flies |
| title_short | A homeostatic clock sets daughter centriole size in flies |
| title_sort | homeostatic clock sets daughter centriole size in flies |
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