Preparative HPLC separation of underivatized amino acids for isotopic analysis
<p>Single-compound analysis of stable or radioactive isotopes has found application in a number of fields ranging from archaeology to forensics. Often, the most difficult part of these analyses is the development of a method for isolating the compound(s) of interest, which can derive from a wi...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Humana Press
2019
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| _version_ | 1797071343790850048 |
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| author | Tripp, JA Devièse, T McCullagh, JSO |
| author_facet | Tripp, JA Devièse, T McCullagh, JSO |
| author_sort | Tripp, JA |
| collection | OXFORD |
| description | <p>Single-compound analysis of stable or radioactive isotopes has found application in a number of fields ranging from archaeology to forensics. Often, the most difficult part of these analyses is the development of a method for isolating the compound(s) of interest, which can derive from a wide range of sample types including the hair, nails, and bone.</p>
<p>Here we describe three complementary preparative HPLC techniques suitable for separating and isolating amino acids from bone collagen and hair keratin. Using preparative reversed-phase, ion-pair, or mixed-mode chromatography in aqueous carbon-free mobile phases, or those from which carbon can easily be removed, underivatized single amino acids can be isolated and further analyzed using mass spectrometric techniques.</p> |
| first_indexed | 2024-03-06T22:51:51Z |
| format | Journal article |
| id | oxford-uuid:5f0f0c4f-6192-4560-bb32-0494c5e61838 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:51:51Z |
| publishDate | 2019 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | oxford-uuid:5f0f0c4f-6192-4560-bb32-0494c5e618382022-03-26T17:44:27ZPreparative HPLC separation of underivatized amino acids for isotopic analysisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5f0f0c4f-6192-4560-bb32-0494c5e61838EnglishSymplectic Elements at OxfordHumana Press2019Tripp, JADevièse, TMcCullagh, JSO<p>Single-compound analysis of stable or radioactive isotopes has found application in a number of fields ranging from archaeology to forensics. Often, the most difficult part of these analyses is the development of a method for isolating the compound(s) of interest, which can derive from a wide range of sample types including the hair, nails, and bone.</p> <p>Here we describe three complementary preparative HPLC techniques suitable for separating and isolating amino acids from bone collagen and hair keratin. Using preparative reversed-phase, ion-pair, or mixed-mode chromatography in aqueous carbon-free mobile phases, or those from which carbon can easily be removed, underivatized single amino acids can be isolated and further analyzed using mass spectrometric techniques.</p> |
| spellingShingle | Tripp, JA Devièse, T McCullagh, JSO Preparative HPLC separation of underivatized amino acids for isotopic analysis |
| title | Preparative HPLC separation of underivatized amino acids for isotopic analysis |
| title_full | Preparative HPLC separation of underivatized amino acids for isotopic analysis |
| title_fullStr | Preparative HPLC separation of underivatized amino acids for isotopic analysis |
| title_full_unstemmed | Preparative HPLC separation of underivatized amino acids for isotopic analysis |
| title_short | Preparative HPLC separation of underivatized amino acids for isotopic analysis |
| title_sort | preparative hplc separation of underivatized amino acids for isotopic analysis |
| work_keys_str_mv | AT trippja preparativehplcseparationofunderivatizedaminoacidsforisotopicanalysis AT devieset preparativehplcseparationofunderivatizedaminoacidsforisotopicanalysis AT mccullaghjso preparativehplcseparationofunderivatizedaminoacidsforisotopicanalysis |