Photoaffinity labelling of isopenicillin N synthetase by laser-flash photolysis.
Isopenicillin N synthetase (IPNS) from Acremonium chrysogenum was photolabelled by laser-flash photolysis in the presence of a diazirinyl-containing substrate, 2-[3-(3-trifluoromethyl-3H-diazirin-3-yl)-phenoxy]acetyl-S- methyloxycarbonylsulphenyl-L-cysteinyl-D-valine (DCV). Labelling of IPNS by DCV...
Main Authors: | Baldwin, J, Coates, J, Halpern, J, Moloney, M, Pratt, A |
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Format: | Journal article |
Language: | English |
Published: |
1989
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