ADP-ribosylation in in vitro systems synthesizing adenovirus DNA.
Two systems utilizing extracts derived from nuclei of adenovirus-infected cells which synthesize adenovirus DNA in vitro were analysed for indications of ADP-ribosylation of virus proteins. On incubation with [32P]NAD or [14C]NAD, modification of the adenovirus T antigen could be demonstrated in one...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1983
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| _version_ | 1826275053754384384 |
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| author | Goding, C Shaw, C Blair, G Russell, W |
| author_facet | Goding, C Shaw, C Blair, G Russell, W |
| author_sort | Goding, C |
| collection | OXFORD |
| description | Two systems utilizing extracts derived from nuclei of adenovirus-infected cells which synthesize adenovirus DNA in vitro were analysed for indications of ADP-ribosylation of virus proteins. On incubation with [32P]NAD or [14C]NAD, modification of the adenovirus T antigen could be demonstrated in one of these systems. ADP-ribosylation of adenovirus core proteins V and VII could also be demonstrated with both nuclear extracts. However, using 3-aminobenzamide, a specific inhibitor of poly(ADP-ribose) polymerase, there was no evidence either in vivo or in vitro that ADP-ribosylation played a critical role in the replication process. |
| first_indexed | 2024-03-06T22:52:51Z |
| format | Journal article |
| id | oxford-uuid:5f5e9420-b63e-4cce-b02e-7440279b8ddc |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:52:51Z |
| publishDate | 1983 |
| record_format | dspace |
| spelling | oxford-uuid:5f5e9420-b63e-4cce-b02e-7440279b8ddc2022-03-26T17:46:37ZADP-ribosylation in in vitro systems synthesizing adenovirus DNA.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5f5e9420-b63e-4cce-b02e-7440279b8ddcEnglishSymplectic Elements at Oxford1983Goding, CShaw, CBlair, GRussell, WTwo systems utilizing extracts derived from nuclei of adenovirus-infected cells which synthesize adenovirus DNA in vitro were analysed for indications of ADP-ribosylation of virus proteins. On incubation with [32P]NAD or [14C]NAD, modification of the adenovirus T antigen could be demonstrated in one of these systems. ADP-ribosylation of adenovirus core proteins V and VII could also be demonstrated with both nuclear extracts. However, using 3-aminobenzamide, a specific inhibitor of poly(ADP-ribose) polymerase, there was no evidence either in vivo or in vitro that ADP-ribosylation played a critical role in the replication process. |
| spellingShingle | Goding, C Shaw, C Blair, G Russell, W ADP-ribosylation in in vitro systems synthesizing adenovirus DNA. |
| title | ADP-ribosylation in in vitro systems synthesizing adenovirus DNA. |
| title_full | ADP-ribosylation in in vitro systems synthesizing adenovirus DNA. |
| title_fullStr | ADP-ribosylation in in vitro systems synthesizing adenovirus DNA. |
| title_full_unstemmed | ADP-ribosylation in in vitro systems synthesizing adenovirus DNA. |
| title_short | ADP-ribosylation in in vitro systems synthesizing adenovirus DNA. |
| title_sort | adp ribosylation in in vitro systems synthesizing adenovirus dna |
| work_keys_str_mv | AT godingc adpribosylationininvitrosystemssynthesizingadenovirusdna AT shawc adpribosylationininvitrosystemssynthesizingadenovirusdna AT blairg adpribosylationininvitrosystemssynthesizingadenovirusdna AT russellw adpribosylationininvitrosystemssynthesizingadenovirusdna |