Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells.
The nonclassical MHC class I molecule human histocompatibility leukocyte antigen (HLA)-G is selectively expressed on fetal trophoblast tissue at the maternal-fetal interface in pregnancy. It has long been suggested that HLA-G may inhibit maternal natural killer (NK) cells through interaction with pa...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1999
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| _version_ | 1797071497209053184 |
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| author | Allan, D Colonna, M Lanier, L Churakova, T Abrams, J Ellis, SA Mcmichael, A Braud, V |
| author_facet | Allan, D Colonna, M Lanier, L Churakova, T Abrams, J Ellis, SA Mcmichael, A Braud, V |
| author_sort | Allan, D |
| collection | OXFORD |
| description | The nonclassical MHC class I molecule human histocompatibility leukocyte antigen (HLA)-G is selectively expressed on fetal trophoblast tissue at the maternal-fetal interface in pregnancy. It has long been suggested that HLA-G may inhibit maternal natural killer (NK) cells through interaction with particular NK cell receptors (KIRs). To investigate interactions of HLA-G, we constructed phycoerythrin-labeled tetrameric complexes of HLA-G refolded with a self-peptide. These HLA-G tetramers failed to bind to NK cells and cells transfected with CD94/NKG2 and killer immunoglobulin-like NK receptors. In contrast, HLA-G tetramers did bind to peripheral blood monocytes, staining a CD16(+)CD14(mid) subset with greater intensity. On transfectants, HLA-G tetramers bound to inhibitory immunoglobulin-like transcript (ILT)2 and ILT4 receptors. However, staining in the presence of antibodies reactive with ILT receptors revealed that the interaction of HLA-G tetramers with blood monocytes was largely due to binding to ILT4. These results suggest that the primary role of HLA-G may be the modulation of myelomonocytic cell behavior in pregnancy. |
| first_indexed | 2024-03-06T22:54:07Z |
| format | Journal article |
| id | oxford-uuid:5fca6bc0-8a61-4eb4-911a-14cfc5cd52eb |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:54:07Z |
| publishDate | 1999 |
| record_format | dspace |
| spelling | oxford-uuid:5fca6bc0-8a61-4eb4-911a-14cfc5cd52eb2022-03-26T17:49:15ZTetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5fca6bc0-8a61-4eb4-911a-14cfc5cd52ebEnglishSymplectic Elements at Oxford1999Allan, DColonna, MLanier, LChurakova, TAbrams, JEllis, SAMcmichael, ABraud, VThe nonclassical MHC class I molecule human histocompatibility leukocyte antigen (HLA)-G is selectively expressed on fetal trophoblast tissue at the maternal-fetal interface in pregnancy. It has long been suggested that HLA-G may inhibit maternal natural killer (NK) cells through interaction with particular NK cell receptors (KIRs). To investigate interactions of HLA-G, we constructed phycoerythrin-labeled tetrameric complexes of HLA-G refolded with a self-peptide. These HLA-G tetramers failed to bind to NK cells and cells transfected with CD94/NKG2 and killer immunoglobulin-like NK receptors. In contrast, HLA-G tetramers did bind to peripheral blood monocytes, staining a CD16(+)CD14(mid) subset with greater intensity. On transfectants, HLA-G tetramers bound to inhibitory immunoglobulin-like transcript (ILT)2 and ILT4 receptors. However, staining in the presence of antibodies reactive with ILT receptors revealed that the interaction of HLA-G tetramers with blood monocytes was largely due to binding to ILT4. These results suggest that the primary role of HLA-G may be the modulation of myelomonocytic cell behavior in pregnancy. |
| spellingShingle | Allan, D Colonna, M Lanier, L Churakova, T Abrams, J Ellis, SA Mcmichael, A Braud, V Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells. |
| title | Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells. |
| title_full | Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells. |
| title_fullStr | Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells. |
| title_full_unstemmed | Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells. |
| title_short | Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells. |
| title_sort | tetrameric complexes of human histocompatibility leukocyte antigen hla g bind to peripheral blood myelomonocytic cells |
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