Phylogenetic analysis of murine leukemia virus sequences from longitudinally sampled chronic fatigue syndrome patients suggests PCR contamination rather than viral evolution.
Xenotropic murine leukemia virus (MLV)-related virus (XMRV) has been amplified from human prostate cancer and chronic fatigue syndrome (CFS) patient samples. Other studies failed to replicate these findings and suggested PCR contamination with a prostate cancer cell line, 22Rv1, as a likely source....
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2011
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| _version_ | 1797071710569103360 |
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| author | Katzourakis, A Hué, S Kellam, P Towers, G |
| author_facet | Katzourakis, A Hué, S Kellam, P Towers, G |
| author_sort | Katzourakis, A |
| collection | OXFORD |
| description | Xenotropic murine leukemia virus (MLV)-related virus (XMRV) has been amplified from human prostate cancer and chronic fatigue syndrome (CFS) patient samples. Other studies failed to replicate these findings and suggested PCR contamination with a prostate cancer cell line, 22Rv1, as a likely source. MLV-like sequences have also been detected in CFS patients in longitudinal samples 15 years apart. Here, we tested whether sequence data from these samples are consistent with viral evolution. Our phylogenetic analyses strongly reject a model of within-patient evolution and demonstrate that the sequences from the first and second time points represent distinct endogenous murine retroviruses, suggesting contamination. |
| first_indexed | 2024-03-06T22:57:15Z |
| format | Journal article |
| id | oxford-uuid:60d9697b-245d-4111-9f97-5970ccaf5ff7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:57:15Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:60d9697b-245d-4111-9f97-5970ccaf5ff72022-03-26T17:55:49ZPhylogenetic analysis of murine leukemia virus sequences from longitudinally sampled chronic fatigue syndrome patients suggests PCR contamination rather than viral evolution.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:60d9697b-245d-4111-9f97-5970ccaf5ff7EnglishSymplectic Elements at Oxford2011Katzourakis, AHué, SKellam, PTowers, GXenotropic murine leukemia virus (MLV)-related virus (XMRV) has been amplified from human prostate cancer and chronic fatigue syndrome (CFS) patient samples. Other studies failed to replicate these findings and suggested PCR contamination with a prostate cancer cell line, 22Rv1, as a likely source. MLV-like sequences have also been detected in CFS patients in longitudinal samples 15 years apart. Here, we tested whether sequence data from these samples are consistent with viral evolution. Our phylogenetic analyses strongly reject a model of within-patient evolution and demonstrate that the sequences from the first and second time points represent distinct endogenous murine retroviruses, suggesting contamination. |
| spellingShingle | Katzourakis, A Hué, S Kellam, P Towers, G Phylogenetic analysis of murine leukemia virus sequences from longitudinally sampled chronic fatigue syndrome patients suggests PCR contamination rather than viral evolution. |
| title | Phylogenetic analysis of murine leukemia virus sequences from longitudinally sampled chronic fatigue syndrome patients suggests PCR contamination rather than viral evolution. |
| title_full | Phylogenetic analysis of murine leukemia virus sequences from longitudinally sampled chronic fatigue syndrome patients suggests PCR contamination rather than viral evolution. |
| title_fullStr | Phylogenetic analysis of murine leukemia virus sequences from longitudinally sampled chronic fatigue syndrome patients suggests PCR contamination rather than viral evolution. |
| title_full_unstemmed | Phylogenetic analysis of murine leukemia virus sequences from longitudinally sampled chronic fatigue syndrome patients suggests PCR contamination rather than viral evolution. |
| title_short | Phylogenetic analysis of murine leukemia virus sequences from longitudinally sampled chronic fatigue syndrome patients suggests PCR contamination rather than viral evolution. |
| title_sort | phylogenetic analysis of murine leukemia virus sequences from longitudinally sampled chronic fatigue syndrome patients suggests pcr contamination rather than viral evolution |
| work_keys_str_mv | AT katzourakisa phylogeneticanalysisofmurineleukemiavirussequencesfromlongitudinallysampledchronicfatiguesyndromepatientssuggestspcrcontaminationratherthanviralevolution AT hues phylogeneticanalysisofmurineleukemiavirussequencesfromlongitudinallysampledchronicfatiguesyndromepatientssuggestspcrcontaminationratherthanviralevolution AT kellamp phylogeneticanalysisofmurineleukemiavirussequencesfromlongitudinallysampledchronicfatiguesyndromepatientssuggestspcrcontaminationratherthanviralevolution AT towersg phylogeneticanalysisofmurineleukemiavirussequencesfromlongitudinallysampledchronicfatiguesyndromepatientssuggestspcrcontaminationratherthanviralevolution |