Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.
Using limiting dilution analysis, we investigated proliferative responses of UCHL1+ and UCHL1- T cell populations to compare the precursor cell frequencies following recall and alloantigen stimulation, and the complexity of cellular interactions within UCHL1+ and UCHL1- populations. We find high fre...
| Principais autores: | , , , |
|---|---|
| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
1988
|
| _version_ | 1826275410328944640 |
|---|---|
| author | Merkenschlager, M Terry, L Edwards, R Beverley, P |
| author_facet | Merkenschlager, M Terry, L Edwards, R Beverley, P |
| author_sort | Merkenschlager, M |
| collection | OXFORD |
| description | Using limiting dilution analysis, we investigated proliferative responses of UCHL1+ and UCHL1- T cell populations to compare the precursor cell frequencies following recall and alloantigen stimulation, and the complexity of cellular interactions within UCHL1+ and UCHL1- populations. We find high frequencies of recall antigen responses among UCHL1+, but not UCHL1- T cells. In contrast, both populations contain similar frequencies of alloantigen responsive cells. Our results are consistent with single-hit kinetics in recall as well as alloantigen responses and show no complex cellular interactions within the responding populations. In conclusion, the difference between the recall antigen response of UCHL1+ and UCHL1- cells observed in conventional proliferation assays is most probably due to a high frequency of recall antigen-responsive UCHL1+ cells and not to suppressive phenomena in the UCHL1- population. These data suggest that memory T cells are largely of the UCHL1+ phenotype. The relation of post-thymic T cell maturation and differential CD45 expression is briefly discussed. |
| first_indexed | 2024-03-06T22:58:16Z |
| format | Journal article |
| id | oxford-uuid:612e3dc8-7f7f-44f0-851a-0a729e594321 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:58:16Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:612e3dc8-7f7f-44f0-851a-0a729e5943212022-03-26T17:58:08ZLimiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:612e3dc8-7f7f-44f0-851a-0a729e594321EnglishSymplectic Elements at Oxford1988Merkenschlager, MTerry, LEdwards, RBeverley, PUsing limiting dilution analysis, we investigated proliferative responses of UCHL1+ and UCHL1- T cell populations to compare the precursor cell frequencies following recall and alloantigen stimulation, and the complexity of cellular interactions within UCHL1+ and UCHL1- populations. We find high frequencies of recall antigen responses among UCHL1+, but not UCHL1- T cells. In contrast, both populations contain similar frequencies of alloantigen responsive cells. Our results are consistent with single-hit kinetics in recall as well as alloantigen responses and show no complex cellular interactions within the responding populations. In conclusion, the difference between the recall antigen response of UCHL1+ and UCHL1- cells observed in conventional proliferation assays is most probably due to a high frequency of recall antigen-responsive UCHL1+ cells and not to suppressive phenomena in the UCHL1- population. These data suggest that memory T cells are largely of the UCHL1+ phenotype. The relation of post-thymic T cell maturation and differential CD45 expression is briefly discussed. |
| spellingShingle | Merkenschlager, M Terry, L Edwards, R Beverley, P Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. |
| title | Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. |
| title_full | Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. |
| title_fullStr | Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. |
| title_full_unstemmed | Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. |
| title_short | Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. |
| title_sort | limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody uchl1 implications for differential cd45 expression in t cell memory formation |
| work_keys_str_mv | AT merkenschlagerm limitingdilutionanalysisofproliferativeresponsesinhumanlymphocytepopulationsdefinedbythemonoclonalantibodyuchl1implicationsfordifferentialcd45expressionintcellmemoryformation AT terryl limitingdilutionanalysisofproliferativeresponsesinhumanlymphocytepopulationsdefinedbythemonoclonalantibodyuchl1implicationsfordifferentialcd45expressionintcellmemoryformation AT edwardsr limitingdilutionanalysisofproliferativeresponsesinhumanlymphocytepopulationsdefinedbythemonoclonalantibodyuchl1implicationsfordifferentialcd45expressionintcellmemoryformation AT beverleyp limitingdilutionanalysisofproliferativeresponsesinhumanlymphocytepopulationsdefinedbythemonoclonalantibodyuchl1implicationsfordifferentialcd45expressionintcellmemoryformation |