Voltage sensitivity of the osteoclast calcium receptor.
We demonstrated previously that osteoclasts possess a divalent cation-sensitive "receptor", the Ca2+ receptor. Activation of the Ca2+ receptor by the surrogate cation Ni2+ was shown to elicit an increase in cytosolic [Ca2+] to a peak value followed by an exponential decline. In the present...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1993
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| _version_ | 1797071817642344448 |
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| author | Pazianas, M Zaidi, M Huang, C Moonga, B Shankar, V |
| author_facet | Pazianas, M Zaidi, M Huang, C Moonga, B Shankar, V |
| author_sort | Pazianas, M |
| collection | OXFORD |
| description | We demonstrated previously that osteoclasts possess a divalent cation-sensitive "receptor", the Ca2+ receptor. Activation of the Ca2+ receptor by the surrogate cation Ni2+ was shown to elicit an increase in cytosolic [Ca2+] to a peak value followed by an exponential decline. In the present study we examined the influence of surface membrane voltage on the kinetics of Ca2+ receptor inactivation. The K+ ionophore, valinomycin was applied to intercept the declining phase of the cytosolic [Ca2+] transient elicited by application of between 50 microM- and 5 mM-[Ni2+]. This resulted in a sustained elevation of cytosolic [Ca2+] or even a 'hump' followed by a gradual decline. Such a kinetic alteration persisted in a Ca(2+)-free solution, but was abolished in high extracellular [K+] (105 mM). Thus, we demonstrate for the first time to our knowledge, a modulatory effect of membrane potential on the function of the osteoclast Ca2+ receptor. |
| first_indexed | 2024-03-06T22:58:42Z |
| format | Journal article |
| id | oxford-uuid:615334ac-c1d0-4622-89bb-a7c3821a0c4a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:58:42Z |
| publishDate | 1993 |
| record_format | dspace |
| spelling | oxford-uuid:615334ac-c1d0-4622-89bb-a7c3821a0c4a2022-03-26T17:59:10ZVoltage sensitivity of the osteoclast calcium receptor.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:615334ac-c1d0-4622-89bb-a7c3821a0c4aEnglishSymplectic Elements at Oxford1993Pazianas, MZaidi, MHuang, CMoonga, BShankar, VWe demonstrated previously that osteoclasts possess a divalent cation-sensitive "receptor", the Ca2+ receptor. Activation of the Ca2+ receptor by the surrogate cation Ni2+ was shown to elicit an increase in cytosolic [Ca2+] to a peak value followed by an exponential decline. In the present study we examined the influence of surface membrane voltage on the kinetics of Ca2+ receptor inactivation. The K+ ionophore, valinomycin was applied to intercept the declining phase of the cytosolic [Ca2+] transient elicited by application of between 50 microM- and 5 mM-[Ni2+]. This resulted in a sustained elevation of cytosolic [Ca2+] or even a 'hump' followed by a gradual decline. Such a kinetic alteration persisted in a Ca(2+)-free solution, but was abolished in high extracellular [K+] (105 mM). Thus, we demonstrate for the first time to our knowledge, a modulatory effect of membrane potential on the function of the osteoclast Ca2+ receptor. |
| spellingShingle | Pazianas, M Zaidi, M Huang, C Moonga, B Shankar, V Voltage sensitivity of the osteoclast calcium receptor. |
| title | Voltage sensitivity of the osteoclast calcium receptor. |
| title_full | Voltage sensitivity of the osteoclast calcium receptor. |
| title_fullStr | Voltage sensitivity of the osteoclast calcium receptor. |
| title_full_unstemmed | Voltage sensitivity of the osteoclast calcium receptor. |
| title_short | Voltage sensitivity of the osteoclast calcium receptor. |
| title_sort | voltage sensitivity of the osteoclast calcium receptor |
| work_keys_str_mv | AT pazianasm voltagesensitivityoftheosteoclastcalciumreceptor AT zaidim voltagesensitivityoftheosteoclastcalciumreceptor AT huangc voltagesensitivityoftheosteoclastcalciumreceptor AT moongab voltagesensitivityoftheosteoclastcalciumreceptor AT shankarv voltagesensitivityoftheosteoclastcalciumreceptor |