Cell type-dependent expression of tubulins in Physarum.
Three alpha-tubulins and two beta-tubulins have been resolved by two-dimensional gel electrophoresis of whole cell lysates of Physarum myxamoebae or plasmodia. Criteria used to identify the tubulins included migration on two-dimensional gels with myxamoebal tubulins purified by self-assembly into mi...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1983
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| _version_ | 1797071865703825408 |
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| author | Burland, T Gull, K Schedl, T Boston, R Dove, W |
| author_facet | Burland, T Gull, K Schedl, T Boston, R Dove, W |
| author_sort | Burland, T |
| collection | OXFORD |
| description | Three alpha-tubulins and two beta-tubulins have been resolved by two-dimensional gel electrophoresis of whole cell lysates of Physarum myxamoebae or plasmodia. Criteria used to identify the tubulins included migration on two-dimensional gels with myxamoebal tubulins purified by self-assembly into microtubules in vitro, peptide mapping with Staphylococcus V8 protease and with chymotrypsin, immunoprecipitation with a monoclonal antibody specific for beta-tubulin, and, finally, hybrid selection of specific mRNA by cloned tubulin DNA sequences, followed by translation in vitro. Differential expression of the Physarum tubulins was observed. The alpha 1- and beta 1-tubulins were detected in both myxamoebae and plasmodia; alpha 2 and beta 2 were detected only in plasmodia, alpha 3 was detected only in the myxamoebal phase, and may be specific to the flagellate. Observation of more tubulin species in plasmodia than in myxamoebae was remarkable; the only microtubules detected in plasmodia are those of the mitotoic spindle, whereas myxamoebae display cytoplasmic, centriolar, flagellar, and mitotic-spindle microtubules. In vitro translation of myxamoebal and plasmodial RNAs indicated that there are distinct mRNAs, and therefore probably separate genes, for the alpha 1-, alpha 2-, beta 1-, and beta 2-tubulins. Thus, the different patterns of tubulin expression in myxamoebae and plasmodia reflect differential expression of tubulin genes. |
| first_indexed | 2024-03-06T22:59:25Z |
| format | Journal article |
| id | oxford-uuid:61919b5b-27b4-45b2-90dd-0afa5ce3260c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:59:25Z |
| publishDate | 1983 |
| record_format | dspace |
| spelling | oxford-uuid:61919b5b-27b4-45b2-90dd-0afa5ce3260c2022-03-26T18:00:53ZCell type-dependent expression of tubulins in Physarum.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:61919b5b-27b4-45b2-90dd-0afa5ce3260cEnglishSymplectic Elements at Oxford1983Burland, TGull, KSchedl, TBoston, RDove, WThree alpha-tubulins and two beta-tubulins have been resolved by two-dimensional gel electrophoresis of whole cell lysates of Physarum myxamoebae or plasmodia. Criteria used to identify the tubulins included migration on two-dimensional gels with myxamoebal tubulins purified by self-assembly into microtubules in vitro, peptide mapping with Staphylococcus V8 protease and with chymotrypsin, immunoprecipitation with a monoclonal antibody specific for beta-tubulin, and, finally, hybrid selection of specific mRNA by cloned tubulin DNA sequences, followed by translation in vitro. Differential expression of the Physarum tubulins was observed. The alpha 1- and beta 1-tubulins were detected in both myxamoebae and plasmodia; alpha 2 and beta 2 were detected only in plasmodia, alpha 3 was detected only in the myxamoebal phase, and may be specific to the flagellate. Observation of more tubulin species in plasmodia than in myxamoebae was remarkable; the only microtubules detected in plasmodia are those of the mitotoic spindle, whereas myxamoebae display cytoplasmic, centriolar, flagellar, and mitotic-spindle microtubules. In vitro translation of myxamoebal and plasmodial RNAs indicated that there are distinct mRNAs, and therefore probably separate genes, for the alpha 1-, alpha 2-, beta 1-, and beta 2-tubulins. Thus, the different patterns of tubulin expression in myxamoebae and plasmodia reflect differential expression of tubulin genes. |
| spellingShingle | Burland, T Gull, K Schedl, T Boston, R Dove, W Cell type-dependent expression of tubulins in Physarum. |
| title | Cell type-dependent expression of tubulins in Physarum. |
| title_full | Cell type-dependent expression of tubulins in Physarum. |
| title_fullStr | Cell type-dependent expression of tubulins in Physarum. |
| title_full_unstemmed | Cell type-dependent expression of tubulins in Physarum. |
| title_short | Cell type-dependent expression of tubulins in Physarum. |
| title_sort | cell type dependent expression of tubulins in physarum |
| work_keys_str_mv | AT burlandt celltypedependentexpressionoftubulinsinphysarum AT gullk celltypedependentexpressionoftubulinsinphysarum AT schedlt celltypedependentexpressionoftubulinsinphysarum AT bostonr celltypedependentexpressionoftubulinsinphysarum AT dovew celltypedependentexpressionoftubulinsinphysarum |