Non-nucleoside inhibitors of mitochondrial thymidine kinase (TK-2) differentially inhibit the closely related herpes simplex virus type 1 TK and Drosophila melanogaster multifunctional deoxynucleoside kinase.
5'-O-Trityl derivatives of thymidine (dThd), (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU), and their acyclic analogs 1-[(Z)-4-triphenylmethoxy-2-butenyl]thymine (KIN-12) and (E)-5-(2-bromovinyl)-1-[(Z)-4-triphenylmethoxy-2-butenyl]uracil (KIN-52) have been synthesized and evaluated for thei...
Päätekijät: | , , , , , , |
---|---|
Aineistotyyppi: | Journal article |
Kieli: | English |
Julkaistu: |
2003
|
_version_ | 1826275500470829056 |
---|---|
author | Balzarini, J Hernández, A Roche, P Esnouf, R Karlsson, A Camarasa, M Pérez-Pérez, M |
author_facet | Balzarini, J Hernández, A Roche, P Esnouf, R Karlsson, A Camarasa, M Pérez-Pérez, M |
author_sort | Balzarini, J |
collection | OXFORD |
description | 5'-O-Trityl derivatives of thymidine (dThd), (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU), and their acyclic analogs 1-[(Z)-4-triphenylmethoxy-2-butenyl]thymine (KIN-12) and (E)-5-(2-bromovinyl)-1-[(Z)-4-triphenylmethoxy-2-butenyl]uracil (KIN-52) have been synthesized and evaluated for their inhibitory activity against the amino acid sequence related mitochondrial dThd kinase (TK-2), herpes simplex virus type 1 (HSV-1) TK, and Drosophila melanogaster multifunctional 2'-deoxynucleoside kinase (Dm-dNK). Several compounds proved markedly inhibitory to these enzymes and represent a new generation of nucleoside kinase inhibitors. KIN-52 was the most potent and selective inhibitor of TK-2 (IC(50), 1.3 microM; K(i), 0.50 microM; K(i)/K(m), 0.37) but was not inhibitory against HSV-1 TK and Dm-dNK at 100 microM. As found for the alternative substrate BVDU, the tritylated compounds competitively inhibited the three enzymes with respect to dThd. However, whereas BVDU behaved as a noncompetitive inhibitor (alternative substrate) of TK-2 and HSV-1 TK with respect to ATP as the varying substrate, the novel tritylated enzyme inhibitors emerged as reversible purely uncompetitive inhibitors of these enzymes. Computer-assisted modeling studies are in agreement with these findings. The tritylated compounds do not act as alternative substrates and they showed a type of kinetics against the nucleoside kinases different from that of BVDU. KIN-12, and particularly KIN-52, are the very first non-nucleoside specific inhibitors of TK-2 reported and may be useful for studying the physiological role of the mitochondrial TK-2 enzyme. |
first_indexed | 2024-03-06T22:59:40Z |
format | Journal article |
id | oxford-uuid:61a453d8-dd7f-453f-b29b-8cae29a5902e |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-06T22:59:40Z |
publishDate | 2003 |
record_format | dspace |
spelling | oxford-uuid:61a453d8-dd7f-453f-b29b-8cae29a5902e2022-03-26T18:01:24ZNon-nucleoside inhibitors of mitochondrial thymidine kinase (TK-2) differentially inhibit the closely related herpes simplex virus type 1 TK and Drosophila melanogaster multifunctional deoxynucleoside kinase.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:61a453d8-dd7f-453f-b29b-8cae29a5902eEnglishSymplectic Elements at Oxford2003Balzarini, JHernández, ARoche, PEsnouf, RKarlsson, ACamarasa, MPérez-Pérez, M5'-O-Trityl derivatives of thymidine (dThd), (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU), and their acyclic analogs 1-[(Z)-4-triphenylmethoxy-2-butenyl]thymine (KIN-12) and (E)-5-(2-bromovinyl)-1-[(Z)-4-triphenylmethoxy-2-butenyl]uracil (KIN-52) have been synthesized and evaluated for their inhibitory activity against the amino acid sequence related mitochondrial dThd kinase (TK-2), herpes simplex virus type 1 (HSV-1) TK, and Drosophila melanogaster multifunctional 2'-deoxynucleoside kinase (Dm-dNK). Several compounds proved markedly inhibitory to these enzymes and represent a new generation of nucleoside kinase inhibitors. KIN-52 was the most potent and selective inhibitor of TK-2 (IC(50), 1.3 microM; K(i), 0.50 microM; K(i)/K(m), 0.37) but was not inhibitory against HSV-1 TK and Dm-dNK at 100 microM. As found for the alternative substrate BVDU, the tritylated compounds competitively inhibited the three enzymes with respect to dThd. However, whereas BVDU behaved as a noncompetitive inhibitor (alternative substrate) of TK-2 and HSV-1 TK with respect to ATP as the varying substrate, the novel tritylated enzyme inhibitors emerged as reversible purely uncompetitive inhibitors of these enzymes. Computer-assisted modeling studies are in agreement with these findings. The tritylated compounds do not act as alternative substrates and they showed a type of kinetics against the nucleoside kinases different from that of BVDU. KIN-12, and particularly KIN-52, are the very first non-nucleoside specific inhibitors of TK-2 reported and may be useful for studying the physiological role of the mitochondrial TK-2 enzyme. |
spellingShingle | Balzarini, J Hernández, A Roche, P Esnouf, R Karlsson, A Camarasa, M Pérez-Pérez, M Non-nucleoside inhibitors of mitochondrial thymidine kinase (TK-2) differentially inhibit the closely related herpes simplex virus type 1 TK and Drosophila melanogaster multifunctional deoxynucleoside kinase. |
title | Non-nucleoside inhibitors of mitochondrial thymidine kinase (TK-2) differentially inhibit the closely related herpes simplex virus type 1 TK and Drosophila melanogaster multifunctional deoxynucleoside kinase. |
title_full | Non-nucleoside inhibitors of mitochondrial thymidine kinase (TK-2) differentially inhibit the closely related herpes simplex virus type 1 TK and Drosophila melanogaster multifunctional deoxynucleoside kinase. |
title_fullStr | Non-nucleoside inhibitors of mitochondrial thymidine kinase (TK-2) differentially inhibit the closely related herpes simplex virus type 1 TK and Drosophila melanogaster multifunctional deoxynucleoside kinase. |
title_full_unstemmed | Non-nucleoside inhibitors of mitochondrial thymidine kinase (TK-2) differentially inhibit the closely related herpes simplex virus type 1 TK and Drosophila melanogaster multifunctional deoxynucleoside kinase. |
title_short | Non-nucleoside inhibitors of mitochondrial thymidine kinase (TK-2) differentially inhibit the closely related herpes simplex virus type 1 TK and Drosophila melanogaster multifunctional deoxynucleoside kinase. |
title_sort | non nucleoside inhibitors of mitochondrial thymidine kinase tk 2 differentially inhibit the closely related herpes simplex virus type 1 tk and drosophila melanogaster multifunctional deoxynucleoside kinase |
work_keys_str_mv | AT balzarinij nonnucleosideinhibitorsofmitochondrialthymidinekinasetk2differentiallyinhibitthecloselyrelatedherpessimplexvirustype1tkanddrosophilamelanogastermultifunctionaldeoxynucleosidekinase AT hernandeza nonnucleosideinhibitorsofmitochondrialthymidinekinasetk2differentiallyinhibitthecloselyrelatedherpessimplexvirustype1tkanddrosophilamelanogastermultifunctionaldeoxynucleosidekinase AT rochep nonnucleosideinhibitorsofmitochondrialthymidinekinasetk2differentiallyinhibitthecloselyrelatedherpessimplexvirustype1tkanddrosophilamelanogastermultifunctionaldeoxynucleosidekinase AT esnoufr nonnucleosideinhibitorsofmitochondrialthymidinekinasetk2differentiallyinhibitthecloselyrelatedherpessimplexvirustype1tkanddrosophilamelanogastermultifunctionaldeoxynucleosidekinase AT karlssona nonnucleosideinhibitorsofmitochondrialthymidinekinasetk2differentiallyinhibitthecloselyrelatedherpessimplexvirustype1tkanddrosophilamelanogastermultifunctionaldeoxynucleosidekinase AT camarasam nonnucleosideinhibitorsofmitochondrialthymidinekinasetk2differentiallyinhibitthecloselyrelatedherpessimplexvirustype1tkanddrosophilamelanogastermultifunctionaldeoxynucleosidekinase AT perezperezm nonnucleosideinhibitorsofmitochondrialthymidinekinasetk2differentiallyinhibitthecloselyrelatedherpessimplexvirustype1tkanddrosophilamelanogastermultifunctionaldeoxynucleosidekinase |