The effects of DNA containing CpG motif on dendritic cells.
Dendritic cells (DC) are specialized antigen-presenting cells. DC can acquire and process antigens in the periphery before maturing and migrating to secondary lymphoid tissues where they present the antigens and deliver co-stimulatory signals to T cells. We describe an immunostimulatory oligonucleot...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2000
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| _version_ | 1826275679309660160 |
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| author | Behboudi, S Chao, D Klenerman, P Austyn, J |
| author_facet | Behboudi, S Chao, D Klenerman, P Austyn, J |
| author_sort | Behboudi, S |
| collection | OXFORD |
| description | Dendritic cells (DC) are specialized antigen-presenting cells. DC can acquire and process antigens in the periphery before maturing and migrating to secondary lymphoid tissues where they present the antigens and deliver co-stimulatory signals to T cells. We describe an immunostimulatory oligonucleotide containing a CpG motif that stimulated murine DC to up-regulate co-stimulatory molecules, induce T-cell proliferative responses and secrete interleukin-12 in vitro. Administration of this oligonucleotide, but not of a control oligonucleotide lacking this motif, to mice led to the disappearance of DC from the marginal zone and T-cell areas of spleen, but not from heart or kidney. The same CpG did not cause maturation of monocyte-derived human DC in vitro, but lipopolysaccharide-treated monocyte-derived DC showed enhanced functional activity and up-regulated co-stimulatory molecules. |
| first_indexed | 2024-03-06T23:02:27Z |
| format | Journal article |
| id | oxford-uuid:629df5a4-4354-4636-b4f6-101c4d0762a2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:02:27Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:629df5a4-4354-4636-b4f6-101c4d0762a22022-03-26T18:07:25ZThe effects of DNA containing CpG motif on dendritic cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:629df5a4-4354-4636-b4f6-101c4d0762a2EnglishSymplectic Elements at Oxford2000Behboudi, SChao, DKlenerman, PAustyn, JDendritic cells (DC) are specialized antigen-presenting cells. DC can acquire and process antigens in the periphery before maturing and migrating to secondary lymphoid tissues where they present the antigens and deliver co-stimulatory signals to T cells. We describe an immunostimulatory oligonucleotide containing a CpG motif that stimulated murine DC to up-regulate co-stimulatory molecules, induce T-cell proliferative responses and secrete interleukin-12 in vitro. Administration of this oligonucleotide, but not of a control oligonucleotide lacking this motif, to mice led to the disappearance of DC from the marginal zone and T-cell areas of spleen, but not from heart or kidney. The same CpG did not cause maturation of monocyte-derived human DC in vitro, but lipopolysaccharide-treated monocyte-derived DC showed enhanced functional activity and up-regulated co-stimulatory molecules. |
| spellingShingle | Behboudi, S Chao, D Klenerman, P Austyn, J The effects of DNA containing CpG motif on dendritic cells. |
| title | The effects of DNA containing CpG motif on dendritic cells. |
| title_full | The effects of DNA containing CpG motif on dendritic cells. |
| title_fullStr | The effects of DNA containing CpG motif on dendritic cells. |
| title_full_unstemmed | The effects of DNA containing CpG motif on dendritic cells. |
| title_short | The effects of DNA containing CpG motif on dendritic cells. |
| title_sort | effects of dna containing cpg motif on dendritic cells |
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