Relationship between novel isoforms, functionally important domains, and subcellular distribution of CD164/endolyn
Functional analyses have indicated that the human CD164 sialomucin may play a key role in hematopoiesis by facilitating the adhesion of human CD34+cells to the stroma and by negatively regulating CD34+CD38lo/− cell proliferation. We have identified three novel human CD164 variants derived by alterna...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
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American Society for Biochemistry and Molecular Biology
2000
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| _version_ | 1797072117083144192 |
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| author | Chan, J Lee-Prudhoe, J Jorgensen, B Ihrke, G Doyonnas, R Zannettino, A Buckle, V Ward, C Simmons, P Watt, S |
| author_facet | Chan, J Lee-Prudhoe, J Jorgensen, B Ihrke, G Doyonnas, R Zannettino, A Buckle, V Ward, C Simmons, P Watt, S |
| author_sort | Chan, J |
| collection | OXFORD |
| description | Functional analyses have indicated that the human CD164 sialomucin may play a key role in hematopoiesis by facilitating the adhesion of human CD34+cells to the stroma and by negatively regulating CD34+CD38lo/− cell proliferation. We have identified three novel human CD164 variants derived by alternative splicing of bona fide exons from a single genomic transcription unit. The predominant CD164(E1–6) isoform, encoded by six exons, is a type I transmembrane protein containing two extracellular mucin domains (I and II) interrupted by a cysteine-rich non-mucin domain. The 103B2/9E10 and 105A5 epitopes, which specify ligand binding characteristics, are located on the exon 1-encoded mucin domain I. Three human CD164(E1–6) mRNA species, exhibiting differential polyadenylation site usage, are differentially expressed in hematopoietic and non-hematopoietic tissues. This study provides additional evidence that human CD164(E1–6) represents the ortholog of murine MGC-24v and rat endolyn. Comparative analysis of murine MGC-24v/CD164(E1–6) with human CD164(E1–6) revealed two potential splice variants and a similar genomic structure. Whereas the human CD164 gene is located on chromosome 6q21, the mouse gene occurs in a syntenic region on chromosome 10B1–B2. By confocal microscopy, human CD164 in CD34+CD38+hematopoietic progenitor (KG1B) and epithelial cell lines appears to be localized primarily in endosomes and lysosomes, with low concentrations at the cell surface. However, in a minority of KG1B cells, CD164 is more prominently expressed at the plasma membrane and in the recycling endosomes, suggesting that its distribution is regulated in cells of hematopoietic origin. |
| first_indexed | 2024-03-06T23:03:01Z |
| format | Journal article |
| id | oxford-uuid:62d30d8a-5b04-4f8d-bf6e-bc846ae08684 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T23:03:01Z |
| publishDate | 2000 |
| publisher | American Society for Biochemistry and Molecular Biology |
| record_format | dspace |
| spelling | oxford-uuid:62d30d8a-5b04-4f8d-bf6e-bc846ae086842022-03-26T18:08:48ZRelationship between novel isoforms, functionally important domains, and subcellular distribution of CD164/endolynJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:62d30d8a-5b04-4f8d-bf6e-bc846ae08684Symplectic Elements at OxfordAmerican Society for Biochemistry and Molecular Biology2000Chan, JLee-Prudhoe, JJorgensen, BIhrke, GDoyonnas, RZannettino, ABuckle, VWard, CSimmons, PWatt, SFunctional analyses have indicated that the human CD164 sialomucin may play a key role in hematopoiesis by facilitating the adhesion of human CD34+cells to the stroma and by negatively regulating CD34+CD38lo/− cell proliferation. We have identified three novel human CD164 variants derived by alternative splicing of bona fide exons from a single genomic transcription unit. The predominant CD164(E1–6) isoform, encoded by six exons, is a type I transmembrane protein containing two extracellular mucin domains (I and II) interrupted by a cysteine-rich non-mucin domain. The 103B2/9E10 and 105A5 epitopes, which specify ligand binding characteristics, are located on the exon 1-encoded mucin domain I. Three human CD164(E1–6) mRNA species, exhibiting differential polyadenylation site usage, are differentially expressed in hematopoietic and non-hematopoietic tissues. This study provides additional evidence that human CD164(E1–6) represents the ortholog of murine MGC-24v and rat endolyn. Comparative analysis of murine MGC-24v/CD164(E1–6) with human CD164(E1–6) revealed two potential splice variants and a similar genomic structure. Whereas the human CD164 gene is located on chromosome 6q21, the mouse gene occurs in a syntenic region on chromosome 10B1–B2. By confocal microscopy, human CD164 in CD34+CD38+hematopoietic progenitor (KG1B) and epithelial cell lines appears to be localized primarily in endosomes and lysosomes, with low concentrations at the cell surface. However, in a minority of KG1B cells, CD164 is more prominently expressed at the plasma membrane and in the recycling endosomes, suggesting that its distribution is regulated in cells of hematopoietic origin. |
| spellingShingle | Chan, J Lee-Prudhoe, J Jorgensen, B Ihrke, G Doyonnas, R Zannettino, A Buckle, V Ward, C Simmons, P Watt, S Relationship between novel isoforms, functionally important domains, and subcellular distribution of CD164/endolyn |
| title | Relationship between novel isoforms, functionally important domains, and subcellular distribution of CD164/endolyn |
| title_full | Relationship between novel isoforms, functionally important domains, and subcellular distribution of CD164/endolyn |
| title_fullStr | Relationship between novel isoforms, functionally important domains, and subcellular distribution of CD164/endolyn |
| title_full_unstemmed | Relationship between novel isoforms, functionally important domains, and subcellular distribution of CD164/endolyn |
| title_short | Relationship between novel isoforms, functionally important domains, and subcellular distribution of CD164/endolyn |
| title_sort | relationship between novel isoforms functionally important domains and subcellular distribution of cd164 endolyn |
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