Structural basis for ligand-dependent dimerization of phenylalanine hydroxylase regulatory domain

The multi-domain enzyme phenylalanine hydroxylase (PAH) catalyzes the hydroxylation of dietary I-phenylalanine (Phe) to I-tyrosine. Inherited mutations that result in PAH enzyme deficiency are the genetic cause of the autosomal recessive disorder phenylketonuria. Phe is the substrate for the PAH act...

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Váldodahkkit: Patel, D, Kopec, J, Fitzpatrick, F, McCorvie, T, Yue, W
Materiálatiipa: Journal article
Almmustuhtton: Nature Publishing Group 2016
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author Patel, D
Kopec, J
Fitzpatrick, F
McCorvie, T
Yue, W
author_facet Patel, D
Kopec, J
Fitzpatrick, F
McCorvie, T
Yue, W
author_sort Patel, D
collection OXFORD
description The multi-domain enzyme phenylalanine hydroxylase (PAH) catalyzes the hydroxylation of dietary I-phenylalanine (Phe) to I-tyrosine. Inherited mutations that result in PAH enzyme deficiency are the genetic cause of the autosomal recessive disorder phenylketonuria. Phe is the substrate for the PAH active site, but also an allosteric ligand that increases enzyme activity. Phe has been proposed to bind, in addition to the catalytic domain, a site at the PAH N-terminal regulatory domain (PAH-RD), to activate the enzyme via an unclear mechanism. Here we report the crystal structure of human PAH-RD bound with Phe at 1.8 Å resolution, revealing a homodimer of ACT folds with Phe bound at the dimer interface. This work delivers the structural evidence to support previous solution studies that a binding site exists in the RD for Phe, and that Phe binding results in dimerization of PAH-RD. Consistent with our structural observation, a disease-associated PAH mutant impaired in Phe binding disrupts the monomer:dimer equilibrium of PAH-RD. Our data therefore support an emerging model of PAH allosteric regulation, whereby Phe binds to PAH-RD and mediates the dimerization of regulatory modules that would bring about conformational changes to activate the enzyme.
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spelling oxford-uuid:62d5d77f-fd7b-4fc6-b0f7-9639c05ce11b2022-03-26T18:08:49ZStructural basis for ligand-dependent dimerization of phenylalanine hydroxylase regulatory domainJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:62d5d77f-fd7b-4fc6-b0f7-9639c05ce11bSymplectic Elements at OxfordNature Publishing Group2016Patel, DKopec, JFitzpatrick, FMcCorvie, TYue, WThe multi-domain enzyme phenylalanine hydroxylase (PAH) catalyzes the hydroxylation of dietary I-phenylalanine (Phe) to I-tyrosine. Inherited mutations that result in PAH enzyme deficiency are the genetic cause of the autosomal recessive disorder phenylketonuria. Phe is the substrate for the PAH active site, but also an allosteric ligand that increases enzyme activity. Phe has been proposed to bind, in addition to the catalytic domain, a site at the PAH N-terminal regulatory domain (PAH-RD), to activate the enzyme via an unclear mechanism. Here we report the crystal structure of human PAH-RD bound with Phe at 1.8 Å resolution, revealing a homodimer of ACT folds with Phe bound at the dimer interface. This work delivers the structural evidence to support previous solution studies that a binding site exists in the RD for Phe, and that Phe binding results in dimerization of PAH-RD. Consistent with our structural observation, a disease-associated PAH mutant impaired in Phe binding disrupts the monomer:dimer equilibrium of PAH-RD. Our data therefore support an emerging model of PAH allosteric regulation, whereby Phe binds to PAH-RD and mediates the dimerization of regulatory modules that would bring about conformational changes to activate the enzyme.
spellingShingle Patel, D
Kopec, J
Fitzpatrick, F
McCorvie, T
Yue, W
Structural basis for ligand-dependent dimerization of phenylalanine hydroxylase regulatory domain
title Structural basis for ligand-dependent dimerization of phenylalanine hydroxylase regulatory domain
title_full Structural basis for ligand-dependent dimerization of phenylalanine hydroxylase regulatory domain
title_fullStr Structural basis for ligand-dependent dimerization of phenylalanine hydroxylase regulatory domain
title_full_unstemmed Structural basis for ligand-dependent dimerization of phenylalanine hydroxylase regulatory domain
title_short Structural basis for ligand-dependent dimerization of phenylalanine hydroxylase regulatory domain
title_sort structural basis for ligand dependent dimerization of phenylalanine hydroxylase regulatory domain
work_keys_str_mv AT pateld structuralbasisforliganddependentdimerizationofphenylalaninehydroxylaseregulatorydomain
AT kopecj structuralbasisforliganddependentdimerizationofphenylalaninehydroxylaseregulatorydomain
AT fitzpatrickf structuralbasisforliganddependentdimerizationofphenylalaninehydroxylaseregulatorydomain
AT mccorviet structuralbasisforliganddependentdimerizationofphenylalaninehydroxylaseregulatorydomain
AT yuew structuralbasisforliganddependentdimerizationofphenylalaninehydroxylaseregulatorydomain