Gene activation properties of a mouse DNA sequence isolated by expression selection.
The MES-1 element was previously isolated from restricted total mouse cellular DNA by "expression selection"--the ability to reactivate expression of a test gene devoid of its 5' enhancer sequences. Mes-1 has been tested in long-term transformation and short-term CAT expression assays...
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1986
|
| _version_ | 1797072127939051520 |
|---|---|
| author | von Hoyningen-Huene, V Norbury, C Griffiths, M Fried, M |
| author_facet | von Hoyningen-Huene, V Norbury, C Griffiths, M Fried, M |
| author_sort | von Hoyningen-Huene, V |
| collection | OXFORD |
| description | The MES-1 element was previously isolated from restricted total mouse cellular DNA by "expression selection"--the ability to reactivate expression of a test gene devoid of its 5' enhancer sequences. Mes-1 has been tested in long-term transformation and short-term CAT expression assays. In both assays MES-1 is active independent of orientation and at a distance when placed 5' to the test gene. The element is active with heterologous promoters and functions efficiently in both rat and mouse cells. MES-1 activates expression by increasing transcription from the test gene's own start (cap) site. Thus the expression selection technique can be used for the isolation of DNA sequences with enhancer-like properties from total cellular DNA. |
| first_indexed | 2024-03-06T23:03:11Z |
| format | Journal article |
| id | oxford-uuid:62e14dfd-31f4-4798-8d0a-a56ad36543e7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:03:11Z |
| publishDate | 1986 |
| record_format | dspace |
| spelling | oxford-uuid:62e14dfd-31f4-4798-8d0a-a56ad36543e72022-03-26T18:09:11ZGene activation properties of a mouse DNA sequence isolated by expression selection.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:62e14dfd-31f4-4798-8d0a-a56ad36543e7EnglishSymplectic Elements at Oxford1986von Hoyningen-Huene, VNorbury, CGriffiths, MFried, MThe MES-1 element was previously isolated from restricted total mouse cellular DNA by "expression selection"--the ability to reactivate expression of a test gene devoid of its 5' enhancer sequences. Mes-1 has been tested in long-term transformation and short-term CAT expression assays. In both assays MES-1 is active independent of orientation and at a distance when placed 5' to the test gene. The element is active with heterologous promoters and functions efficiently in both rat and mouse cells. MES-1 activates expression by increasing transcription from the test gene's own start (cap) site. Thus the expression selection technique can be used for the isolation of DNA sequences with enhancer-like properties from total cellular DNA. |
| spellingShingle | von Hoyningen-Huene, V Norbury, C Griffiths, M Fried, M Gene activation properties of a mouse DNA sequence isolated by expression selection. |
| title | Gene activation properties of a mouse DNA sequence isolated by expression selection. |
| title_full | Gene activation properties of a mouse DNA sequence isolated by expression selection. |
| title_fullStr | Gene activation properties of a mouse DNA sequence isolated by expression selection. |
| title_full_unstemmed | Gene activation properties of a mouse DNA sequence isolated by expression selection. |
| title_short | Gene activation properties of a mouse DNA sequence isolated by expression selection. |
| title_sort | gene activation properties of a mouse dna sequence isolated by expression selection |
| work_keys_str_mv | AT vonhoyningenhuenev geneactivationpropertiesofamousednasequenceisolatedbyexpressionselection AT norburyc geneactivationpropertiesofamousednasequenceisolatedbyexpressionselection AT griffithsm geneactivationpropertiesofamousednasequenceisolatedbyexpressionselection AT friedm geneactivationpropertiesofamousednasequenceisolatedbyexpressionselection |