Multiparametric analysis of myeloid populations by flow cytometry
Flow cytometry is extensively used for the immune-profiling of leukocytes in tissue during homeostasis and inflammation. The multiparametric power of using fluorescently conjugated antibodies for specific surface and activation markers provides a comprehensive profile of immune cells. This chapter d...
| Main Authors: | , , , |
|---|---|
| Format: | Book section |
| Language: | English |
| Published: |
Humana Press
2018
|
| _version_ | 1797072180149747712 |
|---|---|
| author | Mathie, S Corbin, A Eames, H Udalova, I |
| author_facet | Mathie, S Corbin, A Eames, H Udalova, I |
| author_sort | Mathie, S |
| collection | OXFORD |
| description | Flow cytometry is extensively used for the immune-profiling of leukocytes in tissue during homeostasis and inflammation. The multiparametric power of using fluorescently conjugated antibodies for specific surface and activation markers provides a comprehensive profile of immune cells. This chapter describes the identification and characterization of myeloid populations using flow cytometric analysis in an acute model of resolving inflammation. This model allows the examination of heterogenic populations across different systemic and tissue locations. We describe tissue processing, antibody staining, and analysis, which include a newly described viSNE tool to generate two-dimensional clustering within myeloid populations. We also reference the use of transgenic reporter mice on specific myeloid cells that provides enhanced specificity and profiling when defining myeloid heterogeneity. |
| first_indexed | 2024-03-06T23:03:58Z |
| format | Book section |
| id | oxford-uuid:632741cc-2973-491d-a796-2af844a6f97b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:03:58Z |
| publishDate | 2018 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | oxford-uuid:632741cc-2973-491d-a796-2af844a6f97b2022-03-26T18:11:03ZMultiparametric analysis of myeloid populations by flow cytometryBook sectionhttp://purl.org/coar/resource_type/c_3248uuid:632741cc-2973-491d-a796-2af844a6f97bEnglishSymplectic Elements at OxfordHumana Press2018Mathie, SCorbin, AEames, HUdalova, IFlow cytometry is extensively used for the immune-profiling of leukocytes in tissue during homeostasis and inflammation. The multiparametric power of using fluorescently conjugated antibodies for specific surface and activation markers provides a comprehensive profile of immune cells. This chapter describes the identification and characterization of myeloid populations using flow cytometric analysis in an acute model of resolving inflammation. This model allows the examination of heterogenic populations across different systemic and tissue locations. We describe tissue processing, antibody staining, and analysis, which include a newly described viSNE tool to generate two-dimensional clustering within myeloid populations. We also reference the use of transgenic reporter mice on specific myeloid cells that provides enhanced specificity and profiling when defining myeloid heterogeneity. |
| spellingShingle | Mathie, S Corbin, A Eames, H Udalova, I Multiparametric analysis of myeloid populations by flow cytometry |
| title | Multiparametric analysis of myeloid populations by flow cytometry |
| title_full | Multiparametric analysis of myeloid populations by flow cytometry |
| title_fullStr | Multiparametric analysis of myeloid populations by flow cytometry |
| title_full_unstemmed | Multiparametric analysis of myeloid populations by flow cytometry |
| title_short | Multiparametric analysis of myeloid populations by flow cytometry |
| title_sort | multiparametric analysis of myeloid populations by flow cytometry |
| work_keys_str_mv | AT mathies multiparametricanalysisofmyeloidpopulationsbyflowcytometry AT corbina multiparametricanalysisofmyeloidpopulationsbyflowcytometry AT eamesh multiparametricanalysisofmyeloidpopulationsbyflowcytometry AT udalovai multiparametricanalysisofmyeloidpopulationsbyflowcytometry |