Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells
We report the synthesis of hydroxyl-radical (˙OH) responsive fluorescent probes that utilise the 3,5-dihydroxybenzyl (DHB) functionality. 4-Methylumbeliferone-DHB (Umb-DHB) and resorufin-DHB (Res-DHB) in the presence of ˙OH radicals resulted in significant increases in their respective fluorescent e...
| Главные авторы: | , , , , , , , |
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| Формат: | Journal article |
| Язык: | English |
| Опубликовано: |
Royal Society of Chemistry
2023
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| _version_ | 1826310298757234688 |
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| author | Wong, CCY Sun, L-L Liu, M-J Stride, E Raymond, JL Han, H-H Kwan, J Sedgwick, AC |
| author_facet | Wong, CCY Sun, L-L Liu, M-J Stride, E Raymond, JL Han, H-H Kwan, J Sedgwick, AC |
| author_sort | Wong, CCY |
| collection | OXFORD |
| description | We report the synthesis of hydroxyl-radical (˙OH) responsive fluorescent probes that utilise the 3,5-dihydroxybenzyl (DHB) functionality. 4-Methylumbeliferone-DHB (Umb-DHB) and resorufin-DHB (Res-DHB) in the presence of ˙OH radicals resulted in significant increases in their respective fluorescent emission intensities at 460 nm and 585 nm. The incubation of Res-DHB in HeLa cells followed by therapeutic ultrasound (1 MHz) resulted in a significant increase in fluorescence emission intensity thus permitting the ability to monitor ultrasound-induced ˙OH production in live cells. |
| first_indexed | 2024-03-07T07:49:54Z |
| format | Journal article |
| id | oxford-uuid:63ee9e02-0dff-4f7e-9dac-68b216f15ce5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:49:54Z |
| publishDate | 2023 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:63ee9e02-0dff-4f7e-9dac-68b216f15ce52023-07-07T10:11:22ZFluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cellsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:63ee9e02-0dff-4f7e-9dac-68b216f15ce5EnglishSymplectic ElementsRoyal Society of Chemistry2023Wong, CCYSun, L-LLiu, M-JStride, ERaymond, JLHan, H-HKwan, JSedgwick, ACWe report the synthesis of hydroxyl-radical (˙OH) responsive fluorescent probes that utilise the 3,5-dihydroxybenzyl (DHB) functionality. 4-Methylumbeliferone-DHB (Umb-DHB) and resorufin-DHB (Res-DHB) in the presence of ˙OH radicals resulted in significant increases in their respective fluorescent emission intensities at 460 nm and 585 nm. The incubation of Res-DHB in HeLa cells followed by therapeutic ultrasound (1 MHz) resulted in a significant increase in fluorescence emission intensity thus permitting the ability to monitor ultrasound-induced ˙OH production in live cells. |
| spellingShingle | Wong, CCY Sun, L-L Liu, M-J Stride, E Raymond, JL Han, H-H Kwan, J Sedgwick, AC Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells |
| title | Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells |
| title_full | Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells |
| title_fullStr | Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells |
| title_full_unstemmed | Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells |
| title_short | Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells |
| title_sort | fluorescence based chemical tools for monitoring ultrasound induced hydroxyl radical production in aqueous solution and in cells |
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