Isolation of Leishmania promastigote flagella
Eukaryotic flagella are conserved multifunctional organelles with roles in motility, intercellular interactions and signal transduction. Leishmania possess a single flagellum at all stages of their life cycle. Flagella of promastigote forms in the fly are long and motile, with a canonical 9+2 microt...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Humana Press
2020
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| _version_ | 1797072365843120128 |
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| author | Beneke, T Demay, F Wheeler, RJ Gluenz, E |
| author_facet | Beneke, T Demay, F Wheeler, RJ Gluenz, E |
| author_sort | Beneke, T |
| collection | OXFORD |
| description | Eukaryotic flagella are conserved multifunctional organelles with roles in motility, intercellular interactions and signal transduction. Leishmania possess a single flagellum at all stages of their life cycle. Flagella of promastigote forms in the fly are long and motile, with a canonical 9+2 microtubule axoneme and an extra-axonemal paraflagellar rod (PFR). This protocol describes a simple method for the isolation of Leishmania mexicana promastigote flagella, optimised to yield intact flagella that retain both the cytoskeletal elements (9+2 axoneme and PFR) and the surrounding membrane. The isolated flagella and deflagellated cell bodies are suitable for analysis by electron microscopy, protein mass spectrometry and lipidomics. |
| first_indexed | 2024-03-06T23:06:46Z |
| format | Journal article |
| id | oxford-uuid:64161c05-567a-4797-a5bd-75f8523fc7c4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:06:46Z |
| publishDate | 2020 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | oxford-uuid:64161c05-567a-4797-a5bd-75f8523fc7c42022-03-26T18:16:58ZIsolation of Leishmania promastigote flagellaJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:64161c05-567a-4797-a5bd-75f8523fc7c4EnglishSymplectic Elements at OxfordHumana Press2020Beneke, TDemay, FWheeler, RJGluenz, EEukaryotic flagella are conserved multifunctional organelles with roles in motility, intercellular interactions and signal transduction. Leishmania possess a single flagellum at all stages of their life cycle. Flagella of promastigote forms in the fly are long and motile, with a canonical 9+2 microtubule axoneme and an extra-axonemal paraflagellar rod (PFR). This protocol describes a simple method for the isolation of Leishmania mexicana promastigote flagella, optimised to yield intact flagella that retain both the cytoskeletal elements (9+2 axoneme and PFR) and the surrounding membrane. The isolated flagella and deflagellated cell bodies are suitable for analysis by electron microscopy, protein mass spectrometry and lipidomics. |
| spellingShingle | Beneke, T Demay, F Wheeler, RJ Gluenz, E Isolation of Leishmania promastigote flagella |
| title | Isolation of Leishmania promastigote flagella |
| title_full | Isolation of Leishmania promastigote flagella |
| title_fullStr | Isolation of Leishmania promastigote flagella |
| title_full_unstemmed | Isolation of Leishmania promastigote flagella |
| title_short | Isolation of Leishmania promastigote flagella |
| title_sort | isolation of leishmania promastigote flagella |
| work_keys_str_mv | AT beneket isolationofleishmaniapromastigoteflagella AT demayf isolationofleishmaniapromastigoteflagella AT wheelerrj isolationofleishmaniapromastigoteflagella AT gluenze isolationofleishmaniapromastigoteflagella |