Supporting dataset for multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy Jacopo Antonello, Aurélien Barbotin, Ee Zhuan Chong, Jens Rittscher, and Martin Booth https://doi.org/10.1364/OE.393363 This folder contains the raw data acquired throughout the experiments. The data is...
| Main Authors: | , , , , |
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| Format: | Dataset |
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University of Oxford
2020
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| _version_ | 1797072369918935040 |
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| author | Barbotin, A Antonello, J Barbotin, A Chong, E Z Rittscher, J Booth, M J |
| author2 | Rittscher, J |
| author_facet | Rittscher, J Barbotin, A Antonello, J Barbotin, A Chong, E Z Rittscher, J Booth, M J |
| author_sort | Barbotin, A |
| collection | OXFORD |
| description | Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
Jacopo Antonello, Aurélien Barbotin, Ee Zhuan Chong, Jens Rittscher, and Martin Booth
https://doi.org/10.1364/OE.393363
This folder contains the raw data acquired throughout the experiments. The data is grouped by figure number in the paper.
TIFF files are in OME-TIFF (https://docs.openmicroscopy.org/ome-model/latest/) format and can be opened with FiJi (https://imagej.net/Fiji/Downloads). The legend for the file name is <scan-type>-<modality>-<AO off/on>. So that xy-3dsted-aoon.tif corresponds to an xy scan along the lateral plane, in 3D STED mode, with adaptive optics (AO) correction applied.
AO log files contain the raw data acquired during the aberration correction experiments. These files are in HDF5 (https://www.hdfgroup.org/solutions/hdf5/) format and can be opened with a variety of applications (https://en.wikipedia.org/wiki/Hierarchical_Data_Format) that support HDF5 version 1.10 or later. The legend for the file name is ao<enumeration>-<mode-from>-<mode-to>-<modality>.h5. So that ao4-05-10-zsted.h5 corresponds to the fourth part of the aberration correction, where Zernike modes from 5 to 10 inclusive are corrected in ZSTED mode. The acquired images are stored at the address /stacks/stacks. The first image corresponds to the initial state of the correction. Next, the images necessary for the PN algorithm are collected. In the last image, the correction computed by the PN algorithm is applied. For each image, the corresponding column vector of Zernike coefficients applied by the DM is found at the address /dmlib/control/ZernikeControl/x. |
| first_indexed | 2024-03-06T23:06:49Z |
| format | Dataset |
| id | oxford-uuid:641cbf29-f125-4254-bed8-73622634a835 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T23:06:49Z |
| publishDate | 2020 |
| publisher | University of Oxford |
| record_format | dspace |
| spelling | oxford-uuid:641cbf29-f125-4254-bed8-73622634a8352022-03-26T18:17:00ZSupporting dataset for multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopyDatasethttp://purl.org/coar/resource_type/c_ddb1uuid:641cbf29-f125-4254-bed8-73622634a835ORA DepositUniversity of Oxford2020Barbotin, AAntonello, JBarbotin, AChong, E ZRittscher, JBooth, M JRittscher, JBooth, M JMulti-scale sensorless adaptive optics: application to stimulated emission depletion microscopy Jacopo Antonello, Aurélien Barbotin, Ee Zhuan Chong, Jens Rittscher, and Martin Booth https://doi.org/10.1364/OE.393363 This folder contains the raw data acquired throughout the experiments. The data is grouped by figure number in the paper. TIFF files are in OME-TIFF (https://docs.openmicroscopy.org/ome-model/latest/) format and can be opened with FiJi (https://imagej.net/Fiji/Downloads). The legend for the file name is <scan-type>-<modality>-<AO off/on>. So that xy-3dsted-aoon.tif corresponds to an xy scan along the lateral plane, in 3D STED mode, with adaptive optics (AO) correction applied. AO log files contain the raw data acquired during the aberration correction experiments. These files are in HDF5 (https://www.hdfgroup.org/solutions/hdf5/) format and can be opened with a variety of applications (https://en.wikipedia.org/wiki/Hierarchical_Data_Format) that support HDF5 version 1.10 or later. The legend for the file name is ao<enumeration>-<mode-from>-<mode-to>-<modality>.h5. So that ao4-05-10-zsted.h5 corresponds to the fourth part of the aberration correction, where Zernike modes from 5 to 10 inclusive are corrected in ZSTED mode. The acquired images are stored at the address /stacks/stacks. The first image corresponds to the initial state of the correction. Next, the images necessary for the PN algorithm are collected. In the last image, the correction computed by the PN algorithm is applied. For each image, the corresponding column vector of Zernike coefficients applied by the DM is found at the address /dmlib/control/ZernikeControl/x. |
| spellingShingle | Barbotin, A Antonello, J Barbotin, A Chong, E Z Rittscher, J Booth, M J Supporting dataset for multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title | Supporting dataset for multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title_full | Supporting dataset for multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title_fullStr | Supporting dataset for multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title_full_unstemmed | Supporting dataset for multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title_short | Supporting dataset for multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title_sort | supporting dataset for multi scale sensorless adaptive optics application to stimulated emission depletion microscopy |
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