Permanent lymphoid lines from genetically marked lymphocytes: success with lymphocytes recovered from frozen storage.
Permanent human lymphoid cell lines were established successfully from peripheral blood lymphocytes which had been separated for HL-A typing and stored in liquid nitrogen for two years. Frozen lymphocytes were chosen from two siblings who were homozygous at the LA and FOUR HL-A loci. Thawed lymphocy...
| मुख्य लेखकों: | , , , , |
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| स्वरूप: | Journal article |
| भाषा: | English |
| प्रकाशित: |
1976
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| _version_ | 1826276023270899712 |
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| author | Sly, W Sekhon, G Kennett, R Bodmer, W Bodmer, J |
| author_facet | Sly, W Sekhon, G Kennett, R Bodmer, W Bodmer, J |
| author_sort | Sly, W |
| collection | OXFORD |
| description | Permanent human lymphoid cell lines were established successfully from peripheral blood lymphocytes which had been separated for HL-A typing and stored in liquid nitrogen for two years. Frozen lymphocytes were chosen from two siblings who were homozygous at the LA and FOUR HL-A loci. Thawed lymphocytes were transformed with EB virus produced by the marmoset lymphoid line B95-8. No chromosome abnormalities were seen on karyotypes prepared on cells from the established human lymphoid lines using G and Q banding techniques. HL-A typing showed the expected HL-A antigens plus a considerable number of additional reactions. Separation of lymphocytes and freezing them for possible future use requires a relatively small investment. This method of preserving cells can be applied to patients with interesting genetic disorders or other biochemical markers to provide cells which can be transformed and propagated years later. |
| first_indexed | 2024-03-06T23:07:44Z |
| format | Journal article |
| id | oxford-uuid:646e1740-1df5-4e09-8814-27d7f917bbf9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:07:44Z |
| publishDate | 1976 |
| record_format | dspace |
| spelling | oxford-uuid:646e1740-1df5-4e09-8814-27d7f917bbf92022-03-26T18:18:52ZPermanent lymphoid lines from genetically marked lymphocytes: success with lymphocytes recovered from frozen storage.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:646e1740-1df5-4e09-8814-27d7f917bbf9EnglishSymplectic Elements at Oxford1976Sly, WSekhon, GKennett, RBodmer, WBodmer, JPermanent human lymphoid cell lines were established successfully from peripheral blood lymphocytes which had been separated for HL-A typing and stored in liquid nitrogen for two years. Frozen lymphocytes were chosen from two siblings who were homozygous at the LA and FOUR HL-A loci. Thawed lymphocytes were transformed with EB virus produced by the marmoset lymphoid line B95-8. No chromosome abnormalities were seen on karyotypes prepared on cells from the established human lymphoid lines using G and Q banding techniques. HL-A typing showed the expected HL-A antigens plus a considerable number of additional reactions. Separation of lymphocytes and freezing them for possible future use requires a relatively small investment. This method of preserving cells can be applied to patients with interesting genetic disorders or other biochemical markers to provide cells which can be transformed and propagated years later. |
| spellingShingle | Sly, W Sekhon, G Kennett, R Bodmer, W Bodmer, J Permanent lymphoid lines from genetically marked lymphocytes: success with lymphocytes recovered from frozen storage. |
| title | Permanent lymphoid lines from genetically marked lymphocytes: success with lymphocytes recovered from frozen storage. |
| title_full | Permanent lymphoid lines from genetically marked lymphocytes: success with lymphocytes recovered from frozen storage. |
| title_fullStr | Permanent lymphoid lines from genetically marked lymphocytes: success with lymphocytes recovered from frozen storage. |
| title_full_unstemmed | Permanent lymphoid lines from genetically marked lymphocytes: success with lymphocytes recovered from frozen storage. |
| title_short | Permanent lymphoid lines from genetically marked lymphocytes: success with lymphocytes recovered from frozen storage. |
| title_sort | permanent lymphoid lines from genetically marked lymphocytes success with lymphocytes recovered from frozen storage |
| work_keys_str_mv | AT slyw permanentlymphoidlinesfromgeneticallymarkedlymphocytessuccesswithlymphocytesrecoveredfromfrozenstorage AT sekhong permanentlymphoidlinesfromgeneticallymarkedlymphocytessuccesswithlymphocytesrecoveredfromfrozenstorage AT kennettr permanentlymphoidlinesfromgeneticallymarkedlymphocytessuccesswithlymphocytesrecoveredfromfrozenstorage AT bodmerw permanentlymphoidlinesfromgeneticallymarkedlymphocytessuccesswithlymphocytesrecoveredfromfrozenstorage AT bodmerj permanentlymphoidlinesfromgeneticallymarkedlymphocytessuccesswithlymphocytesrecoveredfromfrozenstorage |