A reversible gene trap collection empowers haploid genetics in human cells.
Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no large-scale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells&...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2013
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| _version_ | 1797072506274709504 |
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| author | Bürckstümmer, T Banning, C Hainzl, P Schobesberger, R Kerzendorfer, C Pauler, F Chen, D Them, N Schischlik, F Rebsamen, M Smida, M Fece de la Cruz, F Lapao, A Liszt, M Eizinger, B Guenzl, P Blomen, V Konopka, T Gapp, B Parapatics, K Maier, B Stöckl, J Fischl, W Salic, S Taba Casari, MR |
| author_facet | Bürckstümmer, T Banning, C Hainzl, P Schobesberger, R Kerzendorfer, C Pauler, F Chen, D Them, N Schischlik, F Rebsamen, M Smida, M Fece de la Cruz, F Lapao, A Liszt, M Eizinger, B Guenzl, P Blomen, V Konopka, T Gapp, B Parapatics, K Maier, B Stöckl, J Fischl, W Salic, S Taba Casari, MR |
| author_sort | Bürckstümmer, T |
| collection | OXFORD |
| description | Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no large-scale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the expressed genome, is DNA-barcoded and allows systematic screens for a wide variety of cellular phenotypes. We examined cellular responses to TNF-α, TGF-β, IFN-γ and TNF-related apoptosis-inducing ligand (TRAIL), to illustrate the value of this unique collection of isogenic human cell lines. |
| first_indexed | 2024-03-06T23:08:40Z |
| format | Journal article |
| id | oxford-uuid:64ba4455-e427-49bf-9273-12e27fa7eaa8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:08:40Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:64ba4455-e427-49bf-9273-12e27fa7eaa82022-03-26T18:20:47ZA reversible gene trap collection empowers haploid genetics in human cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:64ba4455-e427-49bf-9273-12e27fa7eaa8EnglishSymplectic Elements at Oxford2013Bürckstümmer, TBanning, CHainzl, PSchobesberger, RKerzendorfer, CPauler, FChen, DThem, NSchischlik, FRebsamen, MSmida, MFece de la Cruz, FLapao, ALiszt, MEizinger, BGuenzl, PBlomen, VKonopka, TGapp, BParapatics, KMaier, BStöckl, JFischl, WSalic, STaba Casari, MRKnockout collections are invaluable tools for studying model organisms such as yeast. However, there are no large-scale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the expressed genome, is DNA-barcoded and allows systematic screens for a wide variety of cellular phenotypes. We examined cellular responses to TNF-α, TGF-β, IFN-γ and TNF-related apoptosis-inducing ligand (TRAIL), to illustrate the value of this unique collection of isogenic human cell lines. |
| spellingShingle | Bürckstümmer, T Banning, C Hainzl, P Schobesberger, R Kerzendorfer, C Pauler, F Chen, D Them, N Schischlik, F Rebsamen, M Smida, M Fece de la Cruz, F Lapao, A Liszt, M Eizinger, B Guenzl, P Blomen, V Konopka, T Gapp, B Parapatics, K Maier, B Stöckl, J Fischl, W Salic, S Taba Casari, MR A reversible gene trap collection empowers haploid genetics in human cells. |
| title | A reversible gene trap collection empowers haploid genetics in human cells. |
| title_full | A reversible gene trap collection empowers haploid genetics in human cells. |
| title_fullStr | A reversible gene trap collection empowers haploid genetics in human cells. |
| title_full_unstemmed | A reversible gene trap collection empowers haploid genetics in human cells. |
| title_short | A reversible gene trap collection empowers haploid genetics in human cells. |
| title_sort | reversible gene trap collection empowers haploid genetics in human cells |
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