Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH.
We have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair oligopurine.oligopyrimidine sequence, using oligonucleotides that contain combinations of 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine (bis-amino-U, BAU) and 3-methyl-2-aminopyridine (MeP) in place of T an...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2005
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| _version_ | 1826276096977403904 |
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| author | Rusling, D Le Strat, L Powers, V Broughton-Head, V Booth, J Lack, O Brown, T Fox, K |
| author_facet | Rusling, D Le Strat, L Powers, V Broughton-Head, V Booth, J Lack, O Brown, T Fox, K |
| author_sort | Rusling, D |
| collection | OXFORD |
| description | We have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair oligopurine.oligopyrimidine sequence, using oligonucleotides that contain combinations of 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine (bis-amino-U, BAU) and 3-methyl-2-aminopyridine (MeP) in place of T and C, respectively. This combination acts cooperatively to enable high affinity triple helix formation at physiological pH. The affinity depends on the number of substitutions and their arrangement; oligonucleotides in which these analogues are evenly distributed throughout the third strand bind much better than those in which they are clustered together. |
| first_indexed | 2024-03-06T23:08:51Z |
| format | Journal article |
| id | oxford-uuid:64c98bf0-5be0-41e8-b8c8-671e2accc442 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:08:51Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:64c98bf0-5be0-41e8-b8c8-671e2accc4422022-03-26T18:21:07ZCombining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:64c98bf0-5be0-41e8-b8c8-671e2accc442EnglishSymplectic Elements at Oxford2005Rusling, DLe Strat, LPowers, VBroughton-Head, VBooth, JLack, OBrown, TFox, KWe have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair oligopurine.oligopyrimidine sequence, using oligonucleotides that contain combinations of 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine (bis-amino-U, BAU) and 3-methyl-2-aminopyridine (MeP) in place of T and C, respectively. This combination acts cooperatively to enable high affinity triple helix formation at physiological pH. The affinity depends on the number of substitutions and their arrangement; oligonucleotides in which these analogues are evenly distributed throughout the third strand bind much better than those in which they are clustered together. |
| spellingShingle | Rusling, D Le Strat, L Powers, V Broughton-Head, V Booth, J Lack, O Brown, T Fox, K Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH. |
| title | Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH. |
| title_full | Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH. |
| title_fullStr | Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH. |
| title_full_unstemmed | Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH. |
| title_short | Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH. |
| title_sort | combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex forming oligonucleotides at physiological ph |
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