Spatiotemporal modelling of CheY complexes in Escherichia coli chemotaxis.
The chemotaxis pathway of Escherichia coli is one of the best studied and modelled biological signalling pathways. Here we extend existing modelling approaches by explicitly including a description of the formation and subcellular localization of intermediary complexes in the phosphotransfer pathway...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2009
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| _version_ | 1826276134236454912 |
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| author | Tindall, M Porter, S Wadhams, G Maini, P Armitage, J |
| author_facet | Tindall, M Porter, S Wadhams, G Maini, P Armitage, J |
| author_sort | Tindall, M |
| collection | OXFORD |
| description | The chemotaxis pathway of Escherichia coli is one of the best studied and modelled biological signalling pathways. Here we extend existing modelling approaches by explicitly including a description of the formation and subcellular localization of intermediary complexes in the phosphotransfer pathway. The inclusion of these complexes shows that only about 60% of the total output response regulator (CheY) is uncomplexed at any moment and hence free to interact with its target, the flagellar motor. A clear strength of this model is its ability to predict the experimentally observable subcellular localization of CheY throughout a chemotactic response. We have found good agreement between the model output and experimentally determined CheY localization patterns. |
| first_indexed | 2024-03-06T23:09:25Z |
| format | Journal article |
| id | oxford-uuid:64f3b84a-a427-48f8-8b4e-6c6b18d7beb3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:09:25Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:64f3b84a-a427-48f8-8b4e-6c6b18d7beb32022-03-26T18:22:18ZSpatiotemporal modelling of CheY complexes in Escherichia coli chemotaxis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:64f3b84a-a427-48f8-8b4e-6c6b18d7beb3EnglishSymplectic Elements at Oxford2009Tindall, MPorter, SWadhams, GMaini, PArmitage, JThe chemotaxis pathway of Escherichia coli is one of the best studied and modelled biological signalling pathways. Here we extend existing modelling approaches by explicitly including a description of the formation and subcellular localization of intermediary complexes in the phosphotransfer pathway. The inclusion of these complexes shows that only about 60% of the total output response regulator (CheY) is uncomplexed at any moment and hence free to interact with its target, the flagellar motor. A clear strength of this model is its ability to predict the experimentally observable subcellular localization of CheY throughout a chemotactic response. We have found good agreement between the model output and experimentally determined CheY localization patterns. |
| spellingShingle | Tindall, M Porter, S Wadhams, G Maini, P Armitage, J Spatiotemporal modelling of CheY complexes in Escherichia coli chemotaxis. |
| title | Spatiotemporal modelling of CheY complexes in Escherichia coli chemotaxis. |
| title_full | Spatiotemporal modelling of CheY complexes in Escherichia coli chemotaxis. |
| title_fullStr | Spatiotemporal modelling of CheY complexes in Escherichia coli chemotaxis. |
| title_full_unstemmed | Spatiotemporal modelling of CheY complexes in Escherichia coli chemotaxis. |
| title_short | Spatiotemporal modelling of CheY complexes in Escherichia coli chemotaxis. |
| title_sort | spatiotemporal modelling of chey complexes in escherichia coli chemotaxis |
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