High-resolution analysis of cis-acting regulatory networks at the α-globin locus.
We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a com...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2013
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| _version_ | 1826276144168566784 |
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| author | Hughes, J Lower, K Dunham, I Taylor, S De Gobbi, M Sloane-Stanley, J McGowan, S Ragoussis, J Vernimmen, D Gibbons, R Higgs, D |
| author_facet | Hughes, J Lower, K Dunham, I Taylor, S De Gobbi, M Sloane-Stanley, J McGowan, S Ragoussis, J Vernimmen, D Gibbons, R Higgs, D |
| author_sort | Hughes, J |
| collection | OXFORD |
| description | We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the α-globin cluster. |
| first_indexed | 2024-03-06T23:09:34Z |
| format | Journal article |
| id | oxford-uuid:64fe1a9b-9074-4cfa-b946-ce26b103fcea |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:09:34Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:64fe1a9b-9074-4cfa-b946-ce26b103fcea2022-03-26T18:22:39ZHigh-resolution analysis of cis-acting regulatory networks at the α-globin locus.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:64fe1a9b-9074-4cfa-b946-ce26b103fceaEnglishSymplectic Elements at Oxford2013Hughes, JLower, KDunham, ITaylor, SDe Gobbi, MSloane-Stanley, JMcGowan, SRagoussis, JVernimmen, DGibbons, RHiggs, DWe have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the α-globin cluster. |
| spellingShingle | Hughes, J Lower, K Dunham, I Taylor, S De Gobbi, M Sloane-Stanley, J McGowan, S Ragoussis, J Vernimmen, D Gibbons, R Higgs, D High-resolution analysis of cis-acting regulatory networks at the α-globin locus. |
| title | High-resolution analysis of cis-acting regulatory networks at the α-globin locus. |
| title_full | High-resolution analysis of cis-acting regulatory networks at the α-globin locus. |
| title_fullStr | High-resolution analysis of cis-acting regulatory networks at the α-globin locus. |
| title_full_unstemmed | High-resolution analysis of cis-acting regulatory networks at the α-globin locus. |
| title_short | High-resolution analysis of cis-acting regulatory networks at the α-globin locus. |
| title_sort | high resolution analysis of cis acting regulatory networks at the α globin locus |
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