Reverse genetics of Drosophila brain structure and function.
A set of molecular genetic technologies are described, which will have far reaching consequences for the study of brain structure, function and development in Drosophila melanogaster. Site selected mutagenesis (a PCR-based screen for P-element insertion events) allows insertion mutants to be isolate...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1994
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| _version_ | 1826276264121466880 |
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| author | Sentry, J Goodwin, S Milligan, C Duncanson, A Yang, M Kaiser, K |
| author_facet | Sentry, J Goodwin, S Milligan, C Duncanson, A Yang, M Kaiser, K |
| author_sort | Sentry, J |
| collection | OXFORD |
| description | A set of molecular genetic technologies are described, which will have far reaching consequences for the study of brain structure, function and development in Drosophila melanogaster. Site selected mutagenesis (a PCR-based screen for P-element insertion events) allows insertion mutants to be isolated for any cloned gene, and is being used in this laboratory to ask questions about the rolls of particular cellular components in learning and memory. Transposants have been isolated in genes encoding a regulatory (RI) and a catalytic (DCO) subunit of cAMP-dependent protein kinase, and in a gene encoding a Gi-like alpha subunit. The alternative use of I factors is described. The PKA RI homozygous mutants display a significant decrement in initial learning ability. Enhancer-trap strategies, for which the GAL-4 P-element system is particularly convenient, allow the identification of genes expressed in the developing fly brain. Strategies for the efficient detection of such events are described. |
| first_indexed | 2024-03-06T23:11:21Z |
| format | Journal article |
| id | oxford-uuid:6594a1fb-8013-476e-b172-1e780229cd7d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:11:21Z |
| publishDate | 1994 |
| record_format | dspace |
| spelling | oxford-uuid:6594a1fb-8013-476e-b172-1e780229cd7d2022-03-26T18:26:21ZReverse genetics of Drosophila brain structure and function.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6594a1fb-8013-476e-b172-1e780229cd7dEnglishSymplectic Elements at Oxford1994Sentry, JGoodwin, SMilligan, CDuncanson, AYang, MKaiser, KA set of molecular genetic technologies are described, which will have far reaching consequences for the study of brain structure, function and development in Drosophila melanogaster. Site selected mutagenesis (a PCR-based screen for P-element insertion events) allows insertion mutants to be isolated for any cloned gene, and is being used in this laboratory to ask questions about the rolls of particular cellular components in learning and memory. Transposants have been isolated in genes encoding a regulatory (RI) and a catalytic (DCO) subunit of cAMP-dependent protein kinase, and in a gene encoding a Gi-like alpha subunit. The alternative use of I factors is described. The PKA RI homozygous mutants display a significant decrement in initial learning ability. Enhancer-trap strategies, for which the GAL-4 P-element system is particularly convenient, allow the identification of genes expressed in the developing fly brain. Strategies for the efficient detection of such events are described. |
| spellingShingle | Sentry, J Goodwin, S Milligan, C Duncanson, A Yang, M Kaiser, K Reverse genetics of Drosophila brain structure and function. |
| title | Reverse genetics of Drosophila brain structure and function. |
| title_full | Reverse genetics of Drosophila brain structure and function. |
| title_fullStr | Reverse genetics of Drosophila brain structure and function. |
| title_full_unstemmed | Reverse genetics of Drosophila brain structure and function. |
| title_short | Reverse genetics of Drosophila brain structure and function. |
| title_sort | reverse genetics of drosophila brain structure and function |
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