Plasma and memory B-cell kinetics in infants following a primary schedule of CRM 197-conjugated serogroup C meningococcal polysaccharide vaccine.
The induction of persistent protective levels of pathogen-specific antibody is an important goal of immunization against childhood infections. However, antibody persistence is poor after immunization in infancy versus later in life. Serogroup C meningococci (MenC) are an important cause of bacteraem...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2009
|
| _version_ | 1797072742434996224 |
|---|---|
| author | Kelly, D Snape, MD Perrett, K Clutterbuck, E Lewis, S Blanchard Rohner, G Jones, M Yu, L Pollard, A |
| author_facet | Kelly, D Snape, MD Perrett, K Clutterbuck, E Lewis, S Blanchard Rohner, G Jones, M Yu, L Pollard, A |
| author_sort | Kelly, D |
| collection | OXFORD |
| description | The induction of persistent protective levels of pathogen-specific antibody is an important goal of immunization against childhood infections. However, antibody persistence is poor after immunization in infancy versus later in life. Serogroup C meningococci (MenC) are an important cause of bacteraemia and meningitis in children. The use of protein-polysaccharide conjugate vaccines against MenC has been associated with a significant decline in the incidence of invasive disease. However, vaccine effectiveness is negligible by more than 1 year after a three-dose priming series in infancy and corresponds to a rapid decline in antibody following an initial immune response. The cellular mechanisms underlying the generation of persistent antibody in this age group are unclear. An essential prelude to larger studies of peripheral blood B cells is an understanding of B-cell kinetics following immunization. We measured MenC- and diphtheria-specific plasma and memory B-cell kinetics in infants receiving a CRM(197) (cross-reactive material; mutant diphtheria toxoid)-conjugated MenC vaccine at 2, 3 and 4 months of age. Plasma cell responses were more delayed after the first dose when compared with the rapid appearance of plasma cells after the third dose. Memory B cells were detectable at all time-points following the third dose as opposed to the low frequency seen following a first dose. This study provides data on B-cell kinetics following a primary schedule of immunization in young infants upon which to base further studies of the underlying cellular mechanism of humoral immunity. |
| first_indexed | 2024-03-06T23:12:04Z |
| format | Journal article |
| id | oxford-uuid:65d4f361-0953-4d03-b8a1-018ab87fb379 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:12:04Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:65d4f361-0953-4d03-b8a1-018ab87fb3792022-03-26T18:28:02ZPlasma and memory B-cell kinetics in infants following a primary schedule of CRM 197-conjugated serogroup C meningococcal polysaccharide vaccine.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:65d4f361-0953-4d03-b8a1-018ab87fb379EnglishSymplectic Elements at Oxford2009Kelly, DSnape, MDPerrett, KClutterbuck, ELewis, SBlanchard Rohner, GJones, MYu, LPollard, AThe induction of persistent protective levels of pathogen-specific antibody is an important goal of immunization against childhood infections. However, antibody persistence is poor after immunization in infancy versus later in life. Serogroup C meningococci (MenC) are an important cause of bacteraemia and meningitis in children. The use of protein-polysaccharide conjugate vaccines against MenC has been associated with a significant decline in the incidence of invasive disease. However, vaccine effectiveness is negligible by more than 1 year after a three-dose priming series in infancy and corresponds to a rapid decline in antibody following an initial immune response. The cellular mechanisms underlying the generation of persistent antibody in this age group are unclear. An essential prelude to larger studies of peripheral blood B cells is an understanding of B-cell kinetics following immunization. We measured MenC- and diphtheria-specific plasma and memory B-cell kinetics in infants receiving a CRM(197) (cross-reactive material; mutant diphtheria toxoid)-conjugated MenC vaccine at 2, 3 and 4 months of age. Plasma cell responses were more delayed after the first dose when compared with the rapid appearance of plasma cells after the third dose. Memory B cells were detectable at all time-points following the third dose as opposed to the low frequency seen following a first dose. This study provides data on B-cell kinetics following a primary schedule of immunization in young infants upon which to base further studies of the underlying cellular mechanism of humoral immunity. |
| spellingShingle | Kelly, D Snape, MD Perrett, K Clutterbuck, E Lewis, S Blanchard Rohner, G Jones, M Yu, L Pollard, A Plasma and memory B-cell kinetics in infants following a primary schedule of CRM 197-conjugated serogroup C meningococcal polysaccharide vaccine. |
| title | Plasma and memory B-cell kinetics in infants following a primary schedule of CRM 197-conjugated serogroup C meningococcal polysaccharide vaccine. |
| title_full | Plasma and memory B-cell kinetics in infants following a primary schedule of CRM 197-conjugated serogroup C meningococcal polysaccharide vaccine. |
| title_fullStr | Plasma and memory B-cell kinetics in infants following a primary schedule of CRM 197-conjugated serogroup C meningococcal polysaccharide vaccine. |
| title_full_unstemmed | Plasma and memory B-cell kinetics in infants following a primary schedule of CRM 197-conjugated serogroup C meningococcal polysaccharide vaccine. |
| title_short | Plasma and memory B-cell kinetics in infants following a primary schedule of CRM 197-conjugated serogroup C meningococcal polysaccharide vaccine. |
| title_sort | plasma and memory b cell kinetics in infants following a primary schedule of crm 197 conjugated serogroup c meningococcal polysaccharide vaccine |
| work_keys_str_mv | AT kellyd plasmaandmemorybcellkineticsininfantsfollowingaprimaryscheduleofcrm197conjugatedserogroupcmeningococcalpolysaccharidevaccine AT snapemd plasmaandmemorybcellkineticsininfantsfollowingaprimaryscheduleofcrm197conjugatedserogroupcmeningococcalpolysaccharidevaccine AT perrettk plasmaandmemorybcellkineticsininfantsfollowingaprimaryscheduleofcrm197conjugatedserogroupcmeningococcalpolysaccharidevaccine AT clutterbucke plasmaandmemorybcellkineticsininfantsfollowingaprimaryscheduleofcrm197conjugatedserogroupcmeningococcalpolysaccharidevaccine AT lewiss plasmaandmemorybcellkineticsininfantsfollowingaprimaryscheduleofcrm197conjugatedserogroupcmeningococcalpolysaccharidevaccine AT blanchardrohnerg plasmaandmemorybcellkineticsininfantsfollowingaprimaryscheduleofcrm197conjugatedserogroupcmeningococcalpolysaccharidevaccine AT jonesm plasmaandmemorybcellkineticsininfantsfollowingaprimaryscheduleofcrm197conjugatedserogroupcmeningococcalpolysaccharidevaccine AT yul plasmaandmemorybcellkineticsininfantsfollowingaprimaryscheduleofcrm197conjugatedserogroupcmeningococcalpolysaccharidevaccine AT pollarda plasmaandmemorybcellkineticsininfantsfollowingaprimaryscheduleofcrm197conjugatedserogroupcmeningococcalpolysaccharidevaccine |