Dynamic regulation of tissue fluidity controls skin repair during wound healing
During wound healing, different pools of stem cells (SCs) contribute to skin repair. However, how SCs become activated and drive the tissue remodeling essential for skin repair is still poorly understood. Here, by developing a mouse model allowing lineage tracing and basal cell lineage ablation, we...
| Autors principals: | , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Idioma: | English |
| Publicat: |
Cell Press
2024
|
| _version_ | 1826314603230920704 |
|---|---|
| author | Sarate, RM Hochstetter, J Valet, M Hallou, A Song, Y Bansaccal, N Ligare, M Aragona, M Engelman, D Bauduin, A Campàs, O Simons, BD Blanpain, C |
| author_facet | Sarate, RM Hochstetter, J Valet, M Hallou, A Song, Y Bansaccal, N Ligare, M Aragona, M Engelman, D Bauduin, A Campàs, O Simons, BD Blanpain, C |
| author_sort | Sarate, RM |
| collection | OXFORD |
| description | During wound healing, different pools of stem cells (SCs) contribute to skin repair. However, how SCs become activated and drive the tissue remodeling essential for skin repair is still poorly understood. Here, by developing a mouse model allowing lineage tracing and basal cell lineage ablation, we monitor SC fate and tissue dynamics during regeneration using confocal and intravital imaging. Analysis of basal cell rearrangements shows dynamic transitions from a solid-like homeostatic state to a fluid-like state allowing tissue remodeling during repair, as predicted by a minimal mathematical modeling of the spatiotemporal dynamics and fate behavior of basal cells. The basal cell layer progressively returns to a solid-like state with re-epithelialization. Bulk, single-cell RNA, and epigenetic profiling of SCs, together with functional experiments, uncover a common regenerative state regulated by the EGFR/AP1 axis activated during tissue fluidization that is essential for skin SC activation and tissue repair. |
| first_indexed | 2024-09-25T04:34:59Z |
| format | Journal article |
| id | oxford-uuid:65da1279-e81d-430e-9a5c-3ec51b90028c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-09-25T04:34:59Z |
| publishDate | 2024 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:65da1279-e81d-430e-9a5c-3ec51b90028c2024-09-20T10:18:56ZDynamic regulation of tissue fluidity controls skin repair during wound healingJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:65da1279-e81d-430e-9a5c-3ec51b90028cEnglishSymplectic ElementsCell Press2024Sarate, RMHochstetter, JValet, MHallou, ASong, YBansaccal, NLigare, MAragona, MEngelman, DBauduin, ACampàs, OSimons, BDBlanpain, CDuring wound healing, different pools of stem cells (SCs) contribute to skin repair. However, how SCs become activated and drive the tissue remodeling essential for skin repair is still poorly understood. Here, by developing a mouse model allowing lineage tracing and basal cell lineage ablation, we monitor SC fate and tissue dynamics during regeneration using confocal and intravital imaging. Analysis of basal cell rearrangements shows dynamic transitions from a solid-like homeostatic state to a fluid-like state allowing tissue remodeling during repair, as predicted by a minimal mathematical modeling of the spatiotemporal dynamics and fate behavior of basal cells. The basal cell layer progressively returns to a solid-like state with re-epithelialization. Bulk, single-cell RNA, and epigenetic profiling of SCs, together with functional experiments, uncover a common regenerative state regulated by the EGFR/AP1 axis activated during tissue fluidization that is essential for skin SC activation and tissue repair. |
| spellingShingle | Sarate, RM Hochstetter, J Valet, M Hallou, A Song, Y Bansaccal, N Ligare, M Aragona, M Engelman, D Bauduin, A Campàs, O Simons, BD Blanpain, C Dynamic regulation of tissue fluidity controls skin repair during wound healing |
| title | Dynamic regulation of tissue fluidity controls skin repair during wound healing |
| title_full | Dynamic regulation of tissue fluidity controls skin repair during wound healing |
| title_fullStr | Dynamic regulation of tissue fluidity controls skin repair during wound healing |
| title_full_unstemmed | Dynamic regulation of tissue fluidity controls skin repair during wound healing |
| title_short | Dynamic regulation of tissue fluidity controls skin repair during wound healing |
| title_sort | dynamic regulation of tissue fluidity controls skin repair during wound healing |
| work_keys_str_mv | AT saraterm dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT hochstetterj dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT valetm dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT halloua dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT songy dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT bansaccaln dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT ligarem dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT aragonam dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT engelmand dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT bauduina dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT campaso dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT simonsbd dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing AT blanpainc dynamicregulationoftissuefluiditycontrolsskinrepairduringwoundhealing |