Membrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits.
Body count.In a generally applicable approach, the number of subunits in fluorescently-labelled protein complexes has been determined by counting photobleaching steps from individual molecules (see figure). The distribution of steps in the pore-forming toxins α-haemolysin and leukocidin indicate sev...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2007
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| _version_ | 1797072837767331840 |
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| author | Das, S Darshi, M Cheley, S Wallace, M Bayley, H |
| author_facet | Das, S Darshi, M Cheley, S Wallace, M Bayley, H |
| author_sort | Das, S |
| collection | OXFORD |
| description | Body count.In a generally applicable approach, the number of subunits in fluorescently-labelled protein complexes has been determined by counting photobleaching steps from individual molecules (see figure). The distribution of steps in the pore-forming toxins α-haemolysin and leukocidin indicate seven subunits for α-hemolysin, and four LukF and four LukS subunits for leukocidin. (Graph Presented). © 2007 Wiley-VCH Verlag GmbHandCo. KGaA. |
| first_indexed | 2024-03-06T23:13:25Z |
| format | Journal article |
| id | oxford-uuid:664b2b43-df47-4931-b952-a727c42701f8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:13:25Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:664b2b43-df47-4931-b952-a727c42701f82022-03-26T18:30:55ZMembrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:664b2b43-df47-4931-b952-a727c42701f8EnglishSymplectic Elements at Oxford2007Das, SDarshi, MCheley, SWallace, MBayley, HBody count.In a generally applicable approach, the number of subunits in fluorescently-labelled protein complexes has been determined by counting photobleaching steps from individual molecules (see figure). The distribution of steps in the pore-forming toxins α-haemolysin and leukocidin indicate seven subunits for α-hemolysin, and four LukF and four LukS subunits for leukocidin. (Graph Presented). © 2007 Wiley-VCH Verlag GmbHandCo. KGaA. |
| spellingShingle | Das, S Darshi, M Cheley, S Wallace, M Bayley, H Membrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits. |
| title | Membrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits. |
| title_full | Membrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits. |
| title_fullStr | Membrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits. |
| title_full_unstemmed | Membrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits. |
| title_short | Membrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits. |
| title_sort | membrane protein stoichiometry determined from the step wise photobleaching of dye labelled subunits |
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