Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling.
Themis (thymocyte-expressed molecule involved in selection), a member of a family of proteins with unknown functions, is highly conserved among vertebrates. Here we found that Themis had high expression in thymocytes between the pre-T cell antigen receptor (pre-TCR) and positive-selection checkpoint...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2009
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| _version_ | 1797072892079374336 |
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| author | Fu, G Vallée, S Rybakin, V McGuire, M Ampudia, J Brockmeyer, C Salek, M Fallen, P Hoerter, J Munshi, A Huang, Y Hu, J Fox, H Sauer, K Acuto, O Gascoigne, N |
| author_facet | Fu, G Vallée, S Rybakin, V McGuire, M Ampudia, J Brockmeyer, C Salek, M Fallen, P Hoerter, J Munshi, A Huang, Y Hu, J Fox, H Sauer, K Acuto, O Gascoigne, N |
| author_sort | Fu, G |
| collection | OXFORD |
| description | Themis (thymocyte-expressed molecule involved in selection), a member of a family of proteins with unknown functions, is highly conserved among vertebrates. Here we found that Themis had high expression in thymocytes between the pre-T cell antigen receptor (pre-TCR) and positive-selection checkpoints and low expression in mature T cells. Themis-deficient thymocytes showed defective positive selection, which resulted in fewer mature thymocytes. Negative selection was also impaired in Themis-deficient mice. A greater percentage of Themis-deficient T cells had CD4(+)CD25(+)Foxp3(+) regulatory and CD62L(lo)CD44(hi) memory phenotypes than did wild-type T cells. In support of the idea that Themis is involved in TCR signaling, this protein was phosphorylated quickly after TCR stimulation and was needed for optimal TCR-driven calcium mobilization and activation of the kinase Erk. |
| first_indexed | 2024-03-06T23:14:13Z |
| format | Journal article |
| id | oxford-uuid:668beb8b-531e-41de-a673-dd51075d2cd3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:14:13Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:668beb8b-531e-41de-a673-dd51075d2cd32022-03-26T18:32:39ZThemis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:668beb8b-531e-41de-a673-dd51075d2cd3EnglishSymplectic Elements at Oxford2009Fu, GVallée, SRybakin, VMcGuire, MAmpudia, JBrockmeyer, CSalek, MFallen, PHoerter, JMunshi, AHuang, YHu, JFox, HSauer, KAcuto, OGascoigne, NThemis (thymocyte-expressed molecule involved in selection), a member of a family of proteins with unknown functions, is highly conserved among vertebrates. Here we found that Themis had high expression in thymocytes between the pre-T cell antigen receptor (pre-TCR) and positive-selection checkpoints and low expression in mature T cells. Themis-deficient thymocytes showed defective positive selection, which resulted in fewer mature thymocytes. Negative selection was also impaired in Themis-deficient mice. A greater percentage of Themis-deficient T cells had CD4(+)CD25(+)Foxp3(+) regulatory and CD62L(lo)CD44(hi) memory phenotypes than did wild-type T cells. In support of the idea that Themis is involved in TCR signaling, this protein was phosphorylated quickly after TCR stimulation and was needed for optimal TCR-driven calcium mobilization and activation of the kinase Erk. |
| spellingShingle | Fu, G Vallée, S Rybakin, V McGuire, M Ampudia, J Brockmeyer, C Salek, M Fallen, P Hoerter, J Munshi, A Huang, Y Hu, J Fox, H Sauer, K Acuto, O Gascoigne, N Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. |
| title | Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. |
| title_full | Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. |
| title_fullStr | Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. |
| title_full_unstemmed | Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. |
| title_short | Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. |
| title_sort | themis controls thymocyte selection through regulation of t cell antigen receptor mediated signaling |
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