Altered potassium channel distribution and composition in myelinated axons suppresses hyperexcitability following injury
Neuropathic pain following peripheral nerve injury is associated with hyperexcitability in damaged myelinated sensory axons, which begins to normalise over time. We investigated the composition and distribution of shaker-type-potassium channels (Kv1 channels) within the nodal complex of myelinated a...
| Հիմնական հեղինակներ: | , , , , , , , , , , , |
|---|---|
| Ձևաչափ: | Journal article |
| Լեզու: | English |
| Հրապարակվել է: |
eLife Sciences Publications
2016
|
| _version_ | 1826276511639928832 |
|---|---|
| author | Calvo, M Richards, N Schmid, A Barroso, A Zhu, L Ivulic, D Zhu, N Anwandter, P Bhat, M Court, F McMahon, S Bennett, D |
| author_facet | Calvo, M Richards, N Schmid, A Barroso, A Zhu, L Ivulic, D Zhu, N Anwandter, P Bhat, M Court, F McMahon, S Bennett, D |
| author_sort | Calvo, M |
| collection | OXFORD |
| description | Neuropathic pain following peripheral nerve injury is associated with hyperexcitability in damaged myelinated sensory axons, which begins to normalise over time. We investigated the composition and distribution of shaker-type-potassium channels (Kv1 channels) within the nodal complex of myelinated axons following injury. At the neuroma that forms after damage, expression of Kv1.1 and 1.2 (normally localised to the juxtaparanode) was markedly decreased. In contrast Kv1.4 and 1.6, which were hardly detectable in the naïve state, showed increased expression within juxtaparanodes and paranodes following injury, both in rats and humans. Within the dorsal root (a site remote from injury) we noted a redistribution of Kv1-channels towards the paranode. Blockade of Kv1 channels with α-DTX after injury reinstated hyperexcitability of A-fibre axons and enhanced mechanosensitivity. Changes in the molecular composition and distribution of axonal Kv1 channels, therefore represents a protective mechanism to suppress the hyperexcitability of myelinated sensory axons that follows nerve injury. |
| first_indexed | 2024-03-06T23:15:04Z |
| format | Journal article |
| id | oxford-uuid:66d8d579-20b2-4937-b752-cf4a3f2d7a57 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:15:04Z |
| publishDate | 2016 |
| publisher | eLife Sciences Publications |
| record_format | dspace |
| spelling | oxford-uuid:66d8d579-20b2-4937-b752-cf4a3f2d7a572022-03-26T18:34:28ZAltered potassium channel distribution and composition in myelinated axons suppresses hyperexcitability following injuryJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:66d8d579-20b2-4937-b752-cf4a3f2d7a57EnglishSymplectic Elements at OxfordeLife Sciences Publications2016Calvo, MRichards, NSchmid, ABarroso, AZhu, LIvulic, DZhu, NAnwandter, PBhat, MCourt, FMcMahon, SBennett, DNeuropathic pain following peripheral nerve injury is associated with hyperexcitability in damaged myelinated sensory axons, which begins to normalise over time. We investigated the composition and distribution of shaker-type-potassium channels (Kv1 channels) within the nodal complex of myelinated axons following injury. At the neuroma that forms after damage, expression of Kv1.1 and 1.2 (normally localised to the juxtaparanode) was markedly decreased. In contrast Kv1.4 and 1.6, which were hardly detectable in the naïve state, showed increased expression within juxtaparanodes and paranodes following injury, both in rats and humans. Within the dorsal root (a site remote from injury) we noted a redistribution of Kv1-channels towards the paranode. Blockade of Kv1 channels with α-DTX after injury reinstated hyperexcitability of A-fibre axons and enhanced mechanosensitivity. Changes in the molecular composition and distribution of axonal Kv1 channels, therefore represents a protective mechanism to suppress the hyperexcitability of myelinated sensory axons that follows nerve injury. |
| spellingShingle | Calvo, M Richards, N Schmid, A Barroso, A Zhu, L Ivulic, D Zhu, N Anwandter, P Bhat, M Court, F McMahon, S Bennett, D Altered potassium channel distribution and composition in myelinated axons suppresses hyperexcitability following injury |
| title | Altered potassium channel distribution and composition in myelinated axons suppresses hyperexcitability following injury |
| title_full | Altered potassium channel distribution and composition in myelinated axons suppresses hyperexcitability following injury |
| title_fullStr | Altered potassium channel distribution and composition in myelinated axons suppresses hyperexcitability following injury |
| title_full_unstemmed | Altered potassium channel distribution and composition in myelinated axons suppresses hyperexcitability following injury |
| title_short | Altered potassium channel distribution and composition in myelinated axons suppresses hyperexcitability following injury |
| title_sort | altered potassium channel distribution and composition in myelinated axons suppresses hyperexcitability following injury |
| work_keys_str_mv | AT calvom alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury AT richardsn alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury AT schmida alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury AT barrosoa alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury AT zhul alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury AT ivulicd alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury AT zhun alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury AT anwandterp alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury AT bhatm alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury AT courtf alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury AT mcmahons alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury AT bennettd alteredpotassiumchanneldistributionandcompositioninmyelinatedaxonssuppresseshyperexcitabilityfollowinginjury |