Recognition of iron-free siderophores by TonB-dependent iron transporters.
TonB-dependent iron transporters reside in the outer membranes of Gram-negative bacteria, transporting ferric-complexes into the periplasm by a mechanism requiring proton motive force and an integral inner membrane complex, TonB-ExbB-ExbD. Certain TonB-dependent transporters contain an additional do...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2004
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| _version_ | 1797072968890712064 |
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| author | Schalk, I Yue, W Buchanan, S |
| author_facet | Schalk, I Yue, W Buchanan, S |
| author_sort | Schalk, I |
| collection | OXFORD |
| description | TonB-dependent iron transporters reside in the outer membranes of Gram-negative bacteria, transporting ferric-complexes into the periplasm by a mechanism requiring proton motive force and an integral inner membrane complex, TonB-ExbB-ExbD. Certain TonB-dependent transporters contain an additional domain at the N-terminus, which interacts with an inner membrane regulatory protein and a cytoplasmic sigma factor to induce transcription of iron transport genes when a ferric-ligand is bound at the extracellular surface of the transporter. Transport of the ferric-ligand is apparently not necessary for transcription induction. Recent biophysical and crystallographic experiments have shown that this subclass of TonB-dependent iron transporters can bind iron-free ligands, whereas only the ferric-ligands are transported into the periplasm. This review focuses on the ligand binding properties of these transporters and includes a discussion of the biological function of the additional domain, the mechanism of transcription induction and the mechanism of ferric-ligand transport. |
| first_indexed | 2024-03-06T23:15:20Z |
| format | Journal article |
| id | oxford-uuid:66f0c921-7949-4688-afce-240139d530b7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:15:20Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:66f0c921-7949-4688-afce-240139d530b72022-03-26T18:34:57ZRecognition of iron-free siderophores by TonB-dependent iron transporters.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:66f0c921-7949-4688-afce-240139d530b7EnglishSymplectic Elements at Oxford2004Schalk, IYue, WBuchanan, STonB-dependent iron transporters reside in the outer membranes of Gram-negative bacteria, transporting ferric-complexes into the periplasm by a mechanism requiring proton motive force and an integral inner membrane complex, TonB-ExbB-ExbD. Certain TonB-dependent transporters contain an additional domain at the N-terminus, which interacts with an inner membrane regulatory protein and a cytoplasmic sigma factor to induce transcription of iron transport genes when a ferric-ligand is bound at the extracellular surface of the transporter. Transport of the ferric-ligand is apparently not necessary for transcription induction. Recent biophysical and crystallographic experiments have shown that this subclass of TonB-dependent iron transporters can bind iron-free ligands, whereas only the ferric-ligands are transported into the periplasm. This review focuses on the ligand binding properties of these transporters and includes a discussion of the biological function of the additional domain, the mechanism of transcription induction and the mechanism of ferric-ligand transport. |
| spellingShingle | Schalk, I Yue, W Buchanan, S Recognition of iron-free siderophores by TonB-dependent iron transporters. |
| title | Recognition of iron-free siderophores by TonB-dependent iron transporters. |
| title_full | Recognition of iron-free siderophores by TonB-dependent iron transporters. |
| title_fullStr | Recognition of iron-free siderophores by TonB-dependent iron transporters. |
| title_full_unstemmed | Recognition of iron-free siderophores by TonB-dependent iron transporters. |
| title_short | Recognition of iron-free siderophores by TonB-dependent iron transporters. |
| title_sort | recognition of iron free siderophores by tonb dependent iron transporters |
| work_keys_str_mv | AT schalki recognitionofironfreesiderophoresbytonbdependentirontransporters AT yuew recognitionofironfreesiderophoresbytonbdependentirontransporters AT buchanans recognitionofironfreesiderophoresbytonbdependentirontransporters |