Spatiotemporally super-resolved volumetric traction force microscopy
Quantification of mechanical forces is a major challenge across biomedical sciences. Yet such measurements are essential to understanding the role of biomechanics in cell regulation and function. Traction force microscopy remains the most broadly applied force probing technology but typically restri...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
American Chemical Society
2019
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| _version_ | 1797072985069191168 |
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| author | Colin-York, H Javanmardi, Y Barbieri, L Li, D Korobchevskaya, K Guo, Y Hall, C Taylor, A Khuon, S Sheridan, G Chew, T Li, D Moeendarbary, E Fritzsche, M |
| author_facet | Colin-York, H Javanmardi, Y Barbieri, L Li, D Korobchevskaya, K Guo, Y Hall, C Taylor, A Khuon, S Sheridan, G Chew, T Li, D Moeendarbary, E Fritzsche, M |
| author_sort | Colin-York, H |
| collection | OXFORD |
| description | Quantification of mechanical forces is a major challenge across biomedical sciences. Yet such measurements are essential to understanding the role of biomechanics in cell regulation and function. Traction force microscopy remains the most broadly applied force probing technology but typically restricts itself to single-plane two-dimensional quantifications with limited spatiotemporal resolution. Here, we introduce an enhanced force measurement technique combining 3D super-resolution fluorescence structural illumination microscopy and traction force microscopy (3D-SIM-TFM) offering increased spatiotemporal resolution, opening-up unprecedented insights into physiological three-dimensional force production in living cells. |
| first_indexed | 2024-03-06T23:15:34Z |
| format | Journal article |
| id | oxford-uuid:67027259-66ac-4fbc-9afc-cad6f15e0ce0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:15:34Z |
| publishDate | 2019 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:67027259-66ac-4fbc-9afc-cad6f15e0ce02022-03-26T18:35:31ZSpatiotemporally super-resolved volumetric traction force microscopyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:67027259-66ac-4fbc-9afc-cad6f15e0ce0EnglishSymplectic Elements at OxfordAmerican Chemical Society2019Colin-York, HJavanmardi, YBarbieri, LLi, DKorobchevskaya, KGuo, YHall, CTaylor, AKhuon, SSheridan, GChew, TLi, DMoeendarbary, EFritzsche, MQuantification of mechanical forces is a major challenge across biomedical sciences. Yet such measurements are essential to understanding the role of biomechanics in cell regulation and function. Traction force microscopy remains the most broadly applied force probing technology but typically restricts itself to single-plane two-dimensional quantifications with limited spatiotemporal resolution. Here, we introduce an enhanced force measurement technique combining 3D super-resolution fluorescence structural illumination microscopy and traction force microscopy (3D-SIM-TFM) offering increased spatiotemporal resolution, opening-up unprecedented insights into physiological three-dimensional force production in living cells. |
| spellingShingle | Colin-York, H Javanmardi, Y Barbieri, L Li, D Korobchevskaya, K Guo, Y Hall, C Taylor, A Khuon, S Sheridan, G Chew, T Li, D Moeendarbary, E Fritzsche, M Spatiotemporally super-resolved volumetric traction force microscopy |
| title | Spatiotemporally super-resolved volumetric traction force microscopy |
| title_full | Spatiotemporally super-resolved volumetric traction force microscopy |
| title_fullStr | Spatiotemporally super-resolved volumetric traction force microscopy |
| title_full_unstemmed | Spatiotemporally super-resolved volumetric traction force microscopy |
| title_short | Spatiotemporally super-resolved volumetric traction force microscopy |
| title_sort | spatiotemporally super resolved volumetric traction force microscopy |
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