LIPID HEADGROUP HYDRATION STUDIED BY H-2-NMR - A LINK BETWEEN SPECTROSCOPY AND THERMODYNAMICS
The conformational and dynamic response of the phosphatidylcholine headgroup to hydration has been characterized by 2H-NMR measurements of selectively deuterated DOPC at positions α, β, and γ, and to complement the structural results from diffraction studies. Quadrupole splittings (ΔνQ) and spin-lat...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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1994
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| _version_ | 1826276584834727936 |
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| author | Ulrich, A Watts, A |
| author_facet | Ulrich, A Watts, A |
| author_sort | Ulrich, A |
| collection | OXFORD |
| description | The conformational and dynamic response of the phosphatidylcholine headgroup to hydration has been characterized by 2H-NMR measurements of selectively deuterated DOPC at positions α, β, and γ, and to complement the structural results from diffraction studies. Quadrupole splittings (ΔνQ) and spin-lattice relaxation rates (1/T1) were monitored over the range of water/lipid mole ratios from 4 to 100. Their hydration dependence reveals a concerted change in headgroup conformation together with an increase in mobility. Both spectroscopic parameters (ΔνQ and 1/T1) are found to be linearly correlated with the activity of the interbilayer water, and it is thus concluded that the 2H-NMR hydration curves are equivalent to lipid sorption isotherms. Using this thermodynamic correlation, the repulsive hydration force between apposing bilayers can be calculated from the 2H-NMR data and a decay constant is evaluated for DOPC of around 3.5 water molecules per lipid. The characterization of the headgroup properties by 2H-NMR is complemented by recent investigations on interbilayer 2H2O, which demonstrates that the lipid-water interface constitutes a single thermodynamic entity. © 1994. |
| first_indexed | 2024-03-06T23:16:10Z |
| format | Journal article |
| id | oxford-uuid:6732f0c1-c4de-4838-8c31-3b453d910c0b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:16:10Z |
| publishDate | 1994 |
| record_format | dspace |
| spelling | oxford-uuid:6732f0c1-c4de-4838-8c31-3b453d910c0b2022-03-26T18:36:45ZLIPID HEADGROUP HYDRATION STUDIED BY H-2-NMR - A LINK BETWEEN SPECTROSCOPY AND THERMODYNAMICSJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6732f0c1-c4de-4838-8c31-3b453d910c0bEnglishSymplectic Elements at Oxford1994Ulrich, AWatts, AThe conformational and dynamic response of the phosphatidylcholine headgroup to hydration has been characterized by 2H-NMR measurements of selectively deuterated DOPC at positions α, β, and γ, and to complement the structural results from diffraction studies. Quadrupole splittings (ΔνQ) and spin-lattice relaxation rates (1/T1) were monitored over the range of water/lipid mole ratios from 4 to 100. Their hydration dependence reveals a concerted change in headgroup conformation together with an increase in mobility. Both spectroscopic parameters (ΔνQ and 1/T1) are found to be linearly correlated with the activity of the interbilayer water, and it is thus concluded that the 2H-NMR hydration curves are equivalent to lipid sorption isotherms. Using this thermodynamic correlation, the repulsive hydration force between apposing bilayers can be calculated from the 2H-NMR data and a decay constant is evaluated for DOPC of around 3.5 water molecules per lipid. The characterization of the headgroup properties by 2H-NMR is complemented by recent investigations on interbilayer 2H2O, which demonstrates that the lipid-water interface constitutes a single thermodynamic entity. © 1994. |
| spellingShingle | Ulrich, A Watts, A LIPID HEADGROUP HYDRATION STUDIED BY H-2-NMR - A LINK BETWEEN SPECTROSCOPY AND THERMODYNAMICS |
| title | LIPID HEADGROUP HYDRATION STUDIED BY H-2-NMR - A LINK BETWEEN SPECTROSCOPY AND THERMODYNAMICS |
| title_full | LIPID HEADGROUP HYDRATION STUDIED BY H-2-NMR - A LINK BETWEEN SPECTROSCOPY AND THERMODYNAMICS |
| title_fullStr | LIPID HEADGROUP HYDRATION STUDIED BY H-2-NMR - A LINK BETWEEN SPECTROSCOPY AND THERMODYNAMICS |
| title_full_unstemmed | LIPID HEADGROUP HYDRATION STUDIED BY H-2-NMR - A LINK BETWEEN SPECTROSCOPY AND THERMODYNAMICS |
| title_short | LIPID HEADGROUP HYDRATION STUDIED BY H-2-NMR - A LINK BETWEEN SPECTROSCOPY AND THERMODYNAMICS |
| title_sort | lipid headgroup hydration studied by h 2 nmr a link between spectroscopy and thermodynamics |
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