Effects of N-butyldeoxynojirimycin and the Lec3.2.8.1 mutant phenotype on N-glycan processing in Chinese hamster ovary cells: application to glycoprotein crystallization.
Heterologous gene expression in either (1) the glycosylation-defective, mutant Chinese hamster ovary cell line, Lec3.2.8.1, or (2) the presence of the alpha-glucosidase inhibitor, N-butyldeoxynojirimycin facilitates the trimming of N-linked glycans of glycoproteins to single N-acetylglucosamine (Glc...
Main Authors: | Butters, T, Sparks, L, Harlos, K, Ikemizu, S, Stuart, D, Jones, E, Davis, S |
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Format: | Journal article |
Language: | English |
Published: |
1999
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