Altered peptide ligands trigger perforin- rather than Fas-dependent cell lysis.
CTLs lyse Fas-expressing target cells by the concomitant action of a perforin- and a Fas-dependent mechanism. This study analyzed whether target cells pulsed with T cell antagonists and other altered peptide ligands (APLs) were susceptible selectively to only one of these two mechanisms. In vivo and...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1997
|
| _version_ | 1797073228248645632 |
|---|---|
| author | Bachmann, M Ohteki, T Faienza, K Zakarian, A Kägi, D Speiser, D Ohashi, P |
| author_facet | Bachmann, M Ohteki, T Faienza, K Zakarian, A Kägi, D Speiser, D Ohashi, P |
| author_sort | Bachmann, M |
| collection | OXFORD |
| description | CTLs lyse Fas-expressing target cells by the concomitant action of a perforin- and a Fas-dependent mechanism. This study analyzed whether target cells pulsed with T cell antagonists and other altered peptide ligands (APLs) were susceptible selectively to only one of these two mechanisms. In vivo and in vitro activated T cells from transgenic mice expressing a TCR specific for lymphocytic choriomeningitis virus were used as effector cells. To distinguish between perforin- and Fas-dependent cytotoxicity, T cells from normal or perforin-deficient mice were used to lyse peptide-pulsed Fas-positive or Fas-negative target cells. In contrast to previous reports that have shown that APLs selectively induce the Fas-dependent pathway of cytotoxicity, our results demonstrate that target cells pulsed with T cell antagonists and other APLs are lysed predominantly by the perforin-dependent pathway. The contribution of Fas-mediated cytotoxicity was similar for the full agonist and the APLs. Thus, full agonists, partial agonists, and antagonists trigger similar and not distinct pathways of cytotoxicity. |
| first_indexed | 2024-03-06T23:19:06Z |
| format | Journal article |
| id | oxford-uuid:682284ad-0df7-40f3-8cdc-4b2fbd4b9997 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:19:06Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:682284ad-0df7-40f3-8cdc-4b2fbd4b99972022-03-26T18:42:55ZAltered peptide ligands trigger perforin- rather than Fas-dependent cell lysis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:682284ad-0df7-40f3-8cdc-4b2fbd4b9997EnglishSymplectic Elements at Oxford1997Bachmann, MOhteki, TFaienza, KZakarian, AKägi, DSpeiser, DOhashi, PCTLs lyse Fas-expressing target cells by the concomitant action of a perforin- and a Fas-dependent mechanism. This study analyzed whether target cells pulsed with T cell antagonists and other altered peptide ligands (APLs) were susceptible selectively to only one of these two mechanisms. In vivo and in vitro activated T cells from transgenic mice expressing a TCR specific for lymphocytic choriomeningitis virus were used as effector cells. To distinguish between perforin- and Fas-dependent cytotoxicity, T cells from normal or perforin-deficient mice were used to lyse peptide-pulsed Fas-positive or Fas-negative target cells. In contrast to previous reports that have shown that APLs selectively induce the Fas-dependent pathway of cytotoxicity, our results demonstrate that target cells pulsed with T cell antagonists and other APLs are lysed predominantly by the perforin-dependent pathway. The contribution of Fas-mediated cytotoxicity was similar for the full agonist and the APLs. Thus, full agonists, partial agonists, and antagonists trigger similar and not distinct pathways of cytotoxicity. |
| spellingShingle | Bachmann, M Ohteki, T Faienza, K Zakarian, A Kägi, D Speiser, D Ohashi, P Altered peptide ligands trigger perforin- rather than Fas-dependent cell lysis. |
| title | Altered peptide ligands trigger perforin- rather than Fas-dependent cell lysis. |
| title_full | Altered peptide ligands trigger perforin- rather than Fas-dependent cell lysis. |
| title_fullStr | Altered peptide ligands trigger perforin- rather than Fas-dependent cell lysis. |
| title_full_unstemmed | Altered peptide ligands trigger perforin- rather than Fas-dependent cell lysis. |
| title_short | Altered peptide ligands trigger perforin- rather than Fas-dependent cell lysis. |
| title_sort | altered peptide ligands trigger perforin rather than fas dependent cell lysis |
| work_keys_str_mv | AT bachmannm alteredpeptideligandstriggerperforinratherthanfasdependentcelllysis AT ohtekit alteredpeptideligandstriggerperforinratherthanfasdependentcelllysis AT faienzak alteredpeptideligandstriggerperforinratherthanfasdependentcelllysis AT zakariana alteredpeptideligandstriggerperforinratherthanfasdependentcelllysis AT kagid alteredpeptideligandstriggerperforinratherthanfasdependentcelllysis AT speiserd alteredpeptideligandstriggerperforinratherthanfasdependentcelllysis AT ohaship alteredpeptideligandstriggerperforinratherthanfasdependentcelllysis |