Osteoclast-like cells in soft tissue leiomyosarcomas.
Giant cell-rich leiomyosarcoma of soft tissues is an unusual variant of malignant smooth muscle tumor characterized by the presence of numerous multinucleated giant cells (MNGCs). The nature of MNGCs and the cellular mechanisms underlying their accumulation in this tumor are poorly understood. Analy...
| Główni autorzy: | , , , , , , |
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| Format: | Journal article |
| Język: | English |
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2010
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| _version_ | 1826276882761383936 |
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| author | Gibbons, C Sun, S Vlychou, M Kliskey, K Lau, Y Sabokbar, A Athanasou, N |
| author_facet | Gibbons, C Sun, S Vlychou, M Kliskey, K Lau, Y Sabokbar, A Athanasou, N |
| author_sort | Gibbons, C |
| collection | OXFORD |
| description | Giant cell-rich leiomyosarcoma of soft tissues is an unusual variant of malignant smooth muscle tumor characterized by the presence of numerous multinucleated giant cells (MNGCs). The nature of MNGCs and the cellular mechanisms underlying their accumulation in this tumor are poorly understood. Analysis of the expression of osteoclast, macrophage, and smooth muscle markers in two cases of giant cell-rich leiomyosarcoma revealed that the MNGCs in giant cell-rich leiomyosarcoma were negative for smooth muscle markers and that these cells expressed an osteoclast-like phenotype, being positive for CD45, CD68, tartrate-resistant acid phosphatase, and CD51 but negative for CD14 and HLA-DR. Scattered tumor-associated macrophages (TAMs) also expressed this phenotype. Leiomyosarcoma tumor cells strongly reacted for CD51 but were negative for CD14, CD45, and CD68. An analysis of 25 conventional (nongiant cell-containing) leiomyosarcomas found isolated CD68(+) MNGCs in three cases (12%), all of which were grade II/III leiomyosarcomas containing a prominent TAM infiltrate. Leiomyosarcoma-derived TAMs in the presence of receptor activator for nuclear factor kappa B ligand (RANKL) and macrophage colony-stimulating factor were capable of differentiating into osteoclast-like cells capable of resorbing bone. Reverse transcription polymerase chain reaction studies showed that RANKL, osteoprotegerin, and TNF-related apoptosis-inducing ligand were expressed by leiomyosarcoma cells. Our findings indicate that the giant cells found in leiomyosarcomas are osteoclast-like and that they are formed from TAMs by a RANKL-dependent mechanism. |
| first_indexed | 2024-03-06T23:20:32Z |
| format | Journal article |
| id | oxford-uuid:6893b7d2-64df-4037-a80e-4974730187b8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:20:32Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:6893b7d2-64df-4037-a80e-4974730187b82022-03-26T18:45:48ZOsteoclast-like cells in soft tissue leiomyosarcomas.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6893b7d2-64df-4037-a80e-4974730187b8EnglishSymplectic Elements at Oxford2010Gibbons, CSun, SVlychou, MKliskey, KLau, YSabokbar, AAthanasou, NGiant cell-rich leiomyosarcoma of soft tissues is an unusual variant of malignant smooth muscle tumor characterized by the presence of numerous multinucleated giant cells (MNGCs). The nature of MNGCs and the cellular mechanisms underlying their accumulation in this tumor are poorly understood. Analysis of the expression of osteoclast, macrophage, and smooth muscle markers in two cases of giant cell-rich leiomyosarcoma revealed that the MNGCs in giant cell-rich leiomyosarcoma were negative for smooth muscle markers and that these cells expressed an osteoclast-like phenotype, being positive for CD45, CD68, tartrate-resistant acid phosphatase, and CD51 but negative for CD14 and HLA-DR. Scattered tumor-associated macrophages (TAMs) also expressed this phenotype. Leiomyosarcoma tumor cells strongly reacted for CD51 but were negative for CD14, CD45, and CD68. An analysis of 25 conventional (nongiant cell-containing) leiomyosarcomas found isolated CD68(+) MNGCs in three cases (12%), all of which were grade II/III leiomyosarcomas containing a prominent TAM infiltrate. Leiomyosarcoma-derived TAMs in the presence of receptor activator for nuclear factor kappa B ligand (RANKL) and macrophage colony-stimulating factor were capable of differentiating into osteoclast-like cells capable of resorbing bone. Reverse transcription polymerase chain reaction studies showed that RANKL, osteoprotegerin, and TNF-related apoptosis-inducing ligand were expressed by leiomyosarcoma cells. Our findings indicate that the giant cells found in leiomyosarcomas are osteoclast-like and that they are formed from TAMs by a RANKL-dependent mechanism. |
| spellingShingle | Gibbons, C Sun, S Vlychou, M Kliskey, K Lau, Y Sabokbar, A Athanasou, N Osteoclast-like cells in soft tissue leiomyosarcomas. |
| title | Osteoclast-like cells in soft tissue leiomyosarcomas. |
| title_full | Osteoclast-like cells in soft tissue leiomyosarcomas. |
| title_fullStr | Osteoclast-like cells in soft tissue leiomyosarcomas. |
| title_full_unstemmed | Osteoclast-like cells in soft tissue leiomyosarcomas. |
| title_short | Osteoclast-like cells in soft tissue leiomyosarcomas. |
| title_sort | osteoclast like cells in soft tissue leiomyosarcomas |
| work_keys_str_mv | AT gibbonsc osteoclastlikecellsinsofttissueleiomyosarcomas AT suns osteoclastlikecellsinsofttissueleiomyosarcomas AT vlychoum osteoclastlikecellsinsofttissueleiomyosarcomas AT kliskeyk osteoclastlikecellsinsofttissueleiomyosarcomas AT lauy osteoclastlikecellsinsofttissueleiomyosarcomas AT sabokbara osteoclastlikecellsinsofttissueleiomyosarcomas AT athanasoun osteoclastlikecellsinsofttissueleiomyosarcomas |