Voltammetric detection of glutathione; an adsorptive stripping voltammetry approach
A simple, sensitive, and rapid detection of glutathione by cyclic voltammetry using a bare glassy carbon electrode is reported in which glutathione forms a 1:1 complex compound with copper(II) ion. This complex compound is adsorbed onto the electrode surface and undergoes electrochemical oxidation a...
| Asıl Yazarlar: | , , |
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| Materyal Türü: | Journal article |
| Baskı/Yayın Bilgisi: |
Royal Society of Chemistry
2016
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| Özet: | A simple, sensitive, and rapid detection of glutathione by cyclic voltammetry using a bare glassy carbon electrode is reported in which glutathione forms a 1:1 complex compound with copper(II) ion. This complex compound is adsorbed onto the electrode surface and undergoes electrochemical oxidation at a characteristic oxidation potential of ca. - 0.20 V vs standard mercury/mercurous sulphate reference electrode, which is used to detect the glutathione concentration. The linear dynamic range is obtained for a glutathione concentration of 1 µM to 12.5 µM, and the sensitivity is found to be 0.1 ± 0.002 μA μM-1. The low limit of detection (n=3) of 0.14 μM and the precision of 1.8% are achieved using a simple, unmodified electrode. The robustness of the present methodology is demonstrated by the successful quantitative analysis of glutathione in the presence of cysteine. |
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