A simple in situ assay to assess plant-associative bacterial nitrogenase activity
Assessment of plant-associative bacterial nitrogen (N) fixation is crucial for selection and development of elite diazotrophic inoculants that could be used to supply cereal crops with nitrogen in a sustainable manner. Although diazotrophic bacteria possess diverse oxygen tolerance mechanisms, most...
| Hoofdauteurs: | , , , , |
|---|---|
| Formaat: | Journal article |
| Taal: | English |
| Gepubliceerd in: |
Frontiers Media
2021
|
| _version_ | 1826276949295628288 |
|---|---|
| author | Haskett, TL Knights, HE Jorrin, B Mendes, MD Poole, PS |
| author_facet | Haskett, TL Knights, HE Jorrin, B Mendes, MD Poole, PS |
| author_sort | Haskett, TL |
| collection | OXFORD |
| description | Assessment of plant-associative bacterial nitrogen (N) fixation is crucial for selection and development of elite diazotrophic inoculants that could be used to supply cereal crops with nitrogen in a sustainable manner. Although diazotrophic bacteria possess diverse oxygen tolerance mechanisms, most require a sub 21% oxygen environment to achieve optimal stability and function of the N-fixing catalyst nitrogenase. Consequently, assessment of N fixation is routinely carried out on "free-living" bacteria grown in the absence of a host plant and such experiments may not accurately divulge activity in the rhizosphere where the availability and forms of nutrients such as carbon and N, which are key regulators of N fixation, may vary widely. Here, we present a modified <i>in situ</i> acetylene reduction assay (ARA), utilizing the model cereal barley as a host to comparatively assess nitrogenase activity in diazotrophic bacteria. The assay is rapid, highly reproducible, applicable to a broad range of diazotrophs, and can be performed with simple equipment commonly found in most laboratories that investigate plant-microbe interactions. Thus, the assay could serve as a first point of order for high-throughput identification of elite plant-associative diazotrophs. |
| first_indexed | 2024-03-06T23:21:32Z |
| format | Journal article |
| id | oxford-uuid:68e3b4b0-2c8c-4a49-b39a-b3c2fdc03ebf |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:21:32Z |
| publishDate | 2021 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | oxford-uuid:68e3b4b0-2c8c-4a49-b39a-b3c2fdc03ebf2022-03-26T18:48:03ZA simple in situ assay to assess plant-associative bacterial nitrogenase activityJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:68e3b4b0-2c8c-4a49-b39a-b3c2fdc03ebfEnglishSymplectic ElementsFrontiers Media2021Haskett, TLKnights, HEJorrin, BMendes, MDPoole, PSAssessment of plant-associative bacterial nitrogen (N) fixation is crucial for selection and development of elite diazotrophic inoculants that could be used to supply cereal crops with nitrogen in a sustainable manner. Although diazotrophic bacteria possess diverse oxygen tolerance mechanisms, most require a sub 21% oxygen environment to achieve optimal stability and function of the N-fixing catalyst nitrogenase. Consequently, assessment of N fixation is routinely carried out on "free-living" bacteria grown in the absence of a host plant and such experiments may not accurately divulge activity in the rhizosphere where the availability and forms of nutrients such as carbon and N, which are key regulators of N fixation, may vary widely. Here, we present a modified <i>in situ</i> acetylene reduction assay (ARA), utilizing the model cereal barley as a host to comparatively assess nitrogenase activity in diazotrophic bacteria. The assay is rapid, highly reproducible, applicable to a broad range of diazotrophs, and can be performed with simple equipment commonly found in most laboratories that investigate plant-microbe interactions. Thus, the assay could serve as a first point of order for high-throughput identification of elite plant-associative diazotrophs. |
| spellingShingle | Haskett, TL Knights, HE Jorrin, B Mendes, MD Poole, PS A simple in situ assay to assess plant-associative bacterial nitrogenase activity |
| title | A simple in situ assay to assess plant-associative bacterial nitrogenase activity |
| title_full | A simple in situ assay to assess plant-associative bacterial nitrogenase activity |
| title_fullStr | A simple in situ assay to assess plant-associative bacterial nitrogenase activity |
| title_full_unstemmed | A simple in situ assay to assess plant-associative bacterial nitrogenase activity |
| title_short | A simple in situ assay to assess plant-associative bacterial nitrogenase activity |
| title_sort | simple in situ assay to assess plant associative bacterial nitrogenase activity |
| work_keys_str_mv | AT hasketttl asimpleinsituassaytoassessplantassociativebacterialnitrogenaseactivity AT knightshe asimpleinsituassaytoassessplantassociativebacterialnitrogenaseactivity AT jorrinb asimpleinsituassaytoassessplantassociativebacterialnitrogenaseactivity AT mendesmd asimpleinsituassaytoassessplantassociativebacterialnitrogenaseactivity AT pooleps asimpleinsituassaytoassessplantassociativebacterialnitrogenaseactivity AT hasketttl simpleinsituassaytoassessplantassociativebacterialnitrogenaseactivity AT knightshe simpleinsituassaytoassessplantassociativebacterialnitrogenaseactivity AT jorrinb simpleinsituassaytoassessplantassociativebacterialnitrogenaseactivity AT mendesmd simpleinsituassaytoassessplantassociativebacterialnitrogenaseactivity AT pooleps simpleinsituassaytoassessplantassociativebacterialnitrogenaseactivity |