Single-molecule and super-resolution imaging of transcription in living bacteria
In vivo single-molecule and super-resolution techniques are transforming our ability to study transcription as it takes place in its native environment in living cells. This review will detail the methods for imaging single molecules in cells, and the data-analysis tools which can be used to extract...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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Elsevier
2017
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| _version_ | 1797073413188091904 |
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| author | Stracy, M Kapanidis, A |
| author_facet | Stracy, M Kapanidis, A |
| author_sort | Stracy, M |
| collection | OXFORD |
| description | In vivo single-molecule and super-resolution techniques are transforming our ability to study transcription as it takes place in its native environment in living cells. This review will detail the methods for imaging single molecules in cells, and the data-analysis tools which can be used to extract quantitative information on the spatial organization, mobility, and kinetics of the transcription machinery from these experiments. Furthermore, we will highlight studies which have applied these techniques to shed new light on bacterial transcription. |
| first_indexed | 2024-03-06T23:21:47Z |
| format | Journal article |
| id | oxford-uuid:68f81e77-eb6c-43b9-b2e0-af408c1a9582 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:21:47Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:68f81e77-eb6c-43b9-b2e0-af408c1a95822022-03-26T18:48:27ZSingle-molecule and super-resolution imaging of transcription in living bacteriaJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:68f81e77-eb6c-43b9-b2e0-af408c1a9582EnglishSymplectic Elements at OxfordElsevier2017Stracy, MKapanidis, AIn vivo single-molecule and super-resolution techniques are transforming our ability to study transcription as it takes place in its native environment in living cells. This review will detail the methods for imaging single molecules in cells, and the data-analysis tools which can be used to extract quantitative information on the spatial organization, mobility, and kinetics of the transcription machinery from these experiments. Furthermore, we will highlight studies which have applied these techniques to shed new light on bacterial transcription. |
| spellingShingle | Stracy, M Kapanidis, A Single-molecule and super-resolution imaging of transcription in living bacteria |
| title | Single-molecule and super-resolution imaging of transcription in living bacteria |
| title_full | Single-molecule and super-resolution imaging of transcription in living bacteria |
| title_fullStr | Single-molecule and super-resolution imaging of transcription in living bacteria |
| title_full_unstemmed | Single-molecule and super-resolution imaging of transcription in living bacteria |
| title_short | Single-molecule and super-resolution imaging of transcription in living bacteria |
| title_sort | single molecule and super resolution imaging of transcription in living bacteria |
| work_keys_str_mv | AT stracym singlemoleculeandsuperresolutionimagingoftranscriptioninlivingbacteria AT kapanidisa singlemoleculeandsuperresolutionimagingoftranscriptioninlivingbacteria |