Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins.
The emission of light, coupled to exocytosis, can in principle be utilized to monitor the activity of a large number of individual synapses simultaneously. To illustrate this concept, fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP-2/synaptobrevin (which we term "synaptoluci...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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1997
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| _version_ | 1826277028615159808 |
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| author | Miesenböck, G Rothman, J |
| author_facet | Miesenböck, G Rothman, J |
| author_sort | Miesenböck, G |
| collection | OXFORD |
| description | The emission of light, coupled to exocytosis, can in principle be utilized to monitor the activity of a large number of individual synapses simultaneously. To illustrate this concept, fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP-2/synaptobrevin (which we term "synaptolucins") were expressed in cultured hippocampal neurons with the help of viral vectors. Synaptolucins were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with their cognate luciferin, which was added to the extracellular medium. Photon emissions required a depolarizing stimulus, occurred from regions with high synaptic density as ascertained by vital staining of recycling synaptic vesicles, and were sensitive to Ca2+ depletion and clostridial neurotoxins. The method can currently detect exocytosis of the readily releasable pool of synaptic vesicles at a hippocampal synapse, corresponding to about two dozen quanta, but has the potential for greater sensitivity. |
| first_indexed | 2024-03-06T23:22:44Z |
| format | Journal article |
| id | oxford-uuid:694b21cb-9ba5-4b74-9345-4a8496d269e9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:22:44Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:694b21cb-9ba5-4b74-9345-4a8496d269e92022-03-26T18:50:20ZPatterns of synaptic activity in neural networks recorded by light emission from synaptolucins.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:694b21cb-9ba5-4b74-9345-4a8496d269e9EnglishSymplectic Elements at Oxford1997Miesenböck, GRothman, JThe emission of light, coupled to exocytosis, can in principle be utilized to monitor the activity of a large number of individual synapses simultaneously. To illustrate this concept, fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP-2/synaptobrevin (which we term "synaptolucins") were expressed in cultured hippocampal neurons with the help of viral vectors. Synaptolucins were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with their cognate luciferin, which was added to the extracellular medium. Photon emissions required a depolarizing stimulus, occurred from regions with high synaptic density as ascertained by vital staining of recycling synaptic vesicles, and were sensitive to Ca2+ depletion and clostridial neurotoxins. The method can currently detect exocytosis of the readily releasable pool of synaptic vesicles at a hippocampal synapse, corresponding to about two dozen quanta, but has the potential for greater sensitivity. |
| spellingShingle | Miesenböck, G Rothman, J Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins. |
| title | Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins. |
| title_full | Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins. |
| title_fullStr | Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins. |
| title_full_unstemmed | Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins. |
| title_short | Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins. |
| title_sort | patterns of synaptic activity in neural networks recorded by light emission from synaptolucins |
| work_keys_str_mv | AT miesenbockg patternsofsynapticactivityinneuralnetworksrecordedbylightemissionfromsynaptolucins AT rothmanj patternsofsynapticactivityinneuralnetworksrecordedbylightemissionfromsynaptolucins |