The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies.
Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homooligomerisation properties of Sas-6 help establish the ninefold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved, but they all contain a predicted Central Coile...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
eLife Sciences Publications
2015
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| _version_ | 1797073496651595776 |
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| author | Cottee, M Muschalik, N Johnson, S Leveson, J Raff, J Lea, S |
| author_facet | Cottee, M Muschalik, N Johnson, S Leveson, J Raff, J Lea, S |
| author_sort | Cottee, M |
| collection | OXFORD |
| description | Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homooligomerisation properties of Sas-6 help establish the ninefold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved, but they all contain a predicted Central Coiled-Coil Domain (CCCD). Here we show that theDrosophilaAna2 CCCD forms a tetramer, and we solve its structure to 0.8 Å, revealing that it adopts an unusual parallel-coil topology. We also solve the structure of the Drosophila Sas-6 N-terminal domain to 2.9 Å revealing that it forms higher-order oligomers through canonical interactions. Point mutations that perturb Sas-6 or Ana2 homo-oligomerisation in vitro strongly perturb centriole assembly in vivo. Thus, efficient centriole duplication in flies requires the homo-oligomerisation of both Sas-6 and Ana2, and the Ana2 CCCD tetramer structure provides important information on how these proteins might cooperate to form a cartwheel structure. |
| first_indexed | 2024-03-06T23:23:01Z |
| format | Journal article |
| id | oxford-uuid:696adc0b-1371-4b5e-b330-c59f3d808aa4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:23:01Z |
| publishDate | 2015 |
| publisher | eLife Sciences Publications |
| record_format | dspace |
| spelling | oxford-uuid:696adc0b-1371-4b5e-b330-c59f3d808aa42022-03-26T18:51:03ZThe homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:696adc0b-1371-4b5e-b330-c59f3d808aa4EnglishSymplectic Elements at OxfordeLife Sciences Publications2015Cottee, MMuschalik, NJohnson, SLeveson, JRaff, JLea, SSas-6 and Ana2/STIL proteins are required for centriole duplication and the homooligomerisation properties of Sas-6 help establish the ninefold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved, but they all contain a predicted Central Coiled-Coil Domain (CCCD). Here we show that theDrosophilaAna2 CCCD forms a tetramer, and we solve its structure to 0.8 Å, revealing that it adopts an unusual parallel-coil topology. We also solve the structure of the Drosophila Sas-6 N-terminal domain to 2.9 Å revealing that it forms higher-order oligomers through canonical interactions. Point mutations that perturb Sas-6 or Ana2 homo-oligomerisation in vitro strongly perturb centriole assembly in vivo. Thus, efficient centriole duplication in flies requires the homo-oligomerisation of both Sas-6 and Ana2, and the Ana2 CCCD tetramer structure provides important information on how these proteins might cooperate to form a cartwheel structure. |
| spellingShingle | Cottee, M Muschalik, N Johnson, S Leveson, J Raff, J Lea, S The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. |
| title | The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. |
| title_full | The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. |
| title_fullStr | The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. |
| title_full_unstemmed | The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. |
| title_short | The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. |
| title_sort | homo oligomerisation of both sas 6 and ana2 is required for efficient centriole assembly in flies |
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