A mass spectrometry-based hybrid method for structural modeling of protein complexes.
We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2014
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| _version_ | 1826277129854124032 |
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| author | Politis, A Stengel, F Hall, Z Hernández, H Leitner, A Walzthoeni, T Robinson, C Aebersold, R |
| author_facet | Politis, A Stengel, F Hall, Z Hernández, H Leitner, A Walzthoeni, T Robinson, C Aebersold, R |
| author_sort | Politis, A |
| collection | OXFORD |
| description | We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base. |
| first_indexed | 2024-03-06T23:24:15Z |
| format | Journal article |
| id | oxford-uuid:69d4ef8b-e240-4e29-a478-d19be5ed2061 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:24:15Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:69d4ef8b-e240-4e29-a478-d19be5ed20612022-03-26T18:53:27ZA mass spectrometry-based hybrid method for structural modeling of protein complexes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:69d4ef8b-e240-4e29-a478-d19be5ed2061EnglishSymplectic Elements at Oxford2014Politis, AStengel, FHall, ZHernández, HLeitner, AWalzthoeni, TRobinson, CAebersold, RWe describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base. |
| spellingShingle | Politis, A Stengel, F Hall, Z Hernández, H Leitner, A Walzthoeni, T Robinson, C Aebersold, R A mass spectrometry-based hybrid method for structural modeling of protein complexes. |
| title | A mass spectrometry-based hybrid method for structural modeling of protein complexes. |
| title_full | A mass spectrometry-based hybrid method for structural modeling of protein complexes. |
| title_fullStr | A mass spectrometry-based hybrid method for structural modeling of protein complexes. |
| title_full_unstemmed | A mass spectrometry-based hybrid method for structural modeling of protein complexes. |
| title_short | A mass spectrometry-based hybrid method for structural modeling of protein complexes. |
| title_sort | mass spectrometry based hybrid method for structural modeling of protein complexes |
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