Architecture of the major component of the type III secretion system export apparatus.
Type III secretion systems (T3SSs) are bacterial membrane-embedded nanomachines designed to export specifically targeted proteins from the bacterial cytoplasm. Secretion through T3SS is governed by a subset of inner membrane proteins termed the 'export apparatus'. We show that a key member...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2013
|
| _version_ | 1826277202545606656 |
|---|---|
| author | Abrusci, P Vergara-Irigaray, M Johnson, S Beeby, MD Hendrixson, DR Roversi, P Friede, M Deane, J Jensen, G Tang, C Lea, S |
| author_facet | Abrusci, P Vergara-Irigaray, M Johnson, S Beeby, MD Hendrixson, DR Roversi, P Friede, M Deane, J Jensen, G Tang, C Lea, S |
| author_sort | Abrusci, P |
| collection | OXFORD |
| description | Type III secretion systems (T3SSs) are bacterial membrane-embedded nanomachines designed to export specifically targeted proteins from the bacterial cytoplasm. Secretion through T3SS is governed by a subset of inner membrane proteins termed the 'export apparatus'. We show that a key member of the Shigella flexneri export apparatus, MxiA, assembles into a ring essential for secretion in vivo. The ring-forming interfaces are well-conserved in both nonflagellar and flagellar homologs, implying that the ring is an evolutionarily conserved feature in these systems. Electron cryo-tomography revealed a T3SS-associated cytoplasmic torus of size and shape corresponding to those of the MxiA ring aligned to the secretion channel located between the secretion pore and the ATPase complex. This defines the molecular architecture of the dominant component of the export apparatus and allows us to propose a model for the molecular mechanisms controlling secretion. |
| first_indexed | 2024-03-06T23:25:22Z |
| format | Journal article |
| id | oxford-uuid:6a304eb1-f3d2-4742-8172-8665f5274589 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:25:22Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:6a304eb1-f3d2-4742-8172-8665f52745892022-03-26T18:55:54ZArchitecture of the major component of the type III secretion system export apparatus.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6a304eb1-f3d2-4742-8172-8665f5274589EnglishSymplectic Elements at Oxford2013Abrusci, PVergara-Irigaray, MJohnson, SBeeby, MDHendrixson, DRRoversi, PFriede, MDeane, JJensen, GTang, CLea, SType III secretion systems (T3SSs) are bacterial membrane-embedded nanomachines designed to export specifically targeted proteins from the bacterial cytoplasm. Secretion through T3SS is governed by a subset of inner membrane proteins termed the 'export apparatus'. We show that a key member of the Shigella flexneri export apparatus, MxiA, assembles into a ring essential for secretion in vivo. The ring-forming interfaces are well-conserved in both nonflagellar and flagellar homologs, implying that the ring is an evolutionarily conserved feature in these systems. Electron cryo-tomography revealed a T3SS-associated cytoplasmic torus of size and shape corresponding to those of the MxiA ring aligned to the secretion channel located between the secretion pore and the ATPase complex. This defines the molecular architecture of the dominant component of the export apparatus and allows us to propose a model for the molecular mechanisms controlling secretion. |
| spellingShingle | Abrusci, P Vergara-Irigaray, M Johnson, S Beeby, MD Hendrixson, DR Roversi, P Friede, M Deane, J Jensen, G Tang, C Lea, S Architecture of the major component of the type III secretion system export apparatus. |
| title | Architecture of the major component of the type III secretion system export apparatus. |
| title_full | Architecture of the major component of the type III secretion system export apparatus. |
| title_fullStr | Architecture of the major component of the type III secretion system export apparatus. |
| title_full_unstemmed | Architecture of the major component of the type III secretion system export apparatus. |
| title_short | Architecture of the major component of the type III secretion system export apparatus. |
| title_sort | architecture of the major component of the type iii secretion system export apparatus |
| work_keys_str_mv | AT abruscip architectureofthemajorcomponentofthetypeiiisecretionsystemexportapparatus AT vergarairigaraym architectureofthemajorcomponentofthetypeiiisecretionsystemexportapparatus AT johnsons architectureofthemajorcomponentofthetypeiiisecretionsystemexportapparatus AT beebymd architectureofthemajorcomponentofthetypeiiisecretionsystemexportapparatus AT hendrixsondr architectureofthemajorcomponentofthetypeiiisecretionsystemexportapparatus AT roversip architectureofthemajorcomponentofthetypeiiisecretionsystemexportapparatus AT friedem architectureofthemajorcomponentofthetypeiiisecretionsystemexportapparatus AT deanej architectureofthemajorcomponentofthetypeiiisecretionsystemexportapparatus AT jenseng architectureofthemajorcomponentofthetypeiiisecretionsystemexportapparatus AT tangc architectureofthemajorcomponentofthetypeiiisecretionsystemexportapparatus AT leas architectureofthemajorcomponentofthetypeiiisecretionsystemexportapparatus |