Recombinant vaccinia viruses for the characterization of Plasmodium falciparum-specific cytotoxic T lymphocytes: recognition of processed antigen despite limited re-stimulation efficacy.
Cytotoxic T lymphocytes (CTL) have been implicated in immunity to Plasmodium falciparum infection and disease. We have previously described the use of peptides to define malaria-specific CTL epitopes. To determine whether these peptide epitopes are processed intracellularly from the whole antigen we...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1997
|
| _version_ | 1797073708972507136 |
|---|---|
| author | Aidoo, M Lalvani, A Whittle, H Hill, A Robson, K |
| author_facet | Aidoo, M Lalvani, A Whittle, H Hill, A Robson, K |
| author_sort | Aidoo, M |
| collection | OXFORD |
| description | Cytotoxic T lymphocytes (CTL) have been implicated in immunity to Plasmodium falciparum infection and disease. We have previously described the use of peptides to define malaria-specific CTL epitopes. To determine whether these peptide epitopes are processed intracellularly from the whole antigen we have developed recombinant vaccinia viruses (rVV) expressing three malaria antigens: thrombospondin-related adhesive protein (TRAP), Pfs16 and the C-terminal half of liver-stage antigen (LSA)-1. Target cells infected with recombinant viruses were lysed by malaria-specific CTL from semi-immune African donors. We also tested the ability of cells infected with these recombinant vaccinia viruses to re-stimulate malaria-specific CTL in peripheral blood lymphocytes from malaria immune adults. Two other pox virus recombinants, NYVAC, an attenuated vaccinia virus, and ALVAC, a canarypox virus, both expressing malaria antigens were also evaluated for their ability to stimulate malaria-specific CTL in contrast to peptide, none of these viruses successfully re-stimulated CTL from the peripheral blood lymphocytes of semi-immune donors. The ability of human CTL from naturally exposed individuals to recognize processed antigen supports the relevance of these cells in protective immunity to malaria. |
| first_indexed | 2024-03-06T23:25:58Z |
| format | Journal article |
| id | oxford-uuid:6a5f5a4e-2a70-41cf-956b-d60d7ca6db87 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:25:58Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:6a5f5a4e-2a70-41cf-956b-d60d7ca6db872022-03-26T18:57:05ZRecombinant vaccinia viruses for the characterization of Plasmodium falciparum-specific cytotoxic T lymphocytes: recognition of processed antigen despite limited re-stimulation efficacy.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6a5f5a4e-2a70-41cf-956b-d60d7ca6db87EnglishSymplectic Elements at Oxford1997Aidoo, MLalvani, AWhittle, HHill, ARobson, KCytotoxic T lymphocytes (CTL) have been implicated in immunity to Plasmodium falciparum infection and disease. We have previously described the use of peptides to define malaria-specific CTL epitopes. To determine whether these peptide epitopes are processed intracellularly from the whole antigen we have developed recombinant vaccinia viruses (rVV) expressing three malaria antigens: thrombospondin-related adhesive protein (TRAP), Pfs16 and the C-terminal half of liver-stage antigen (LSA)-1. Target cells infected with recombinant viruses were lysed by malaria-specific CTL from semi-immune African donors. We also tested the ability of cells infected with these recombinant vaccinia viruses to re-stimulate malaria-specific CTL in peripheral blood lymphocytes from malaria immune adults. Two other pox virus recombinants, NYVAC, an attenuated vaccinia virus, and ALVAC, a canarypox virus, both expressing malaria antigens were also evaluated for their ability to stimulate malaria-specific CTL in contrast to peptide, none of these viruses successfully re-stimulated CTL from the peripheral blood lymphocytes of semi-immune donors. The ability of human CTL from naturally exposed individuals to recognize processed antigen supports the relevance of these cells in protective immunity to malaria. |
| spellingShingle | Aidoo, M Lalvani, A Whittle, H Hill, A Robson, K Recombinant vaccinia viruses for the characterization of Plasmodium falciparum-specific cytotoxic T lymphocytes: recognition of processed antigen despite limited re-stimulation efficacy. |
| title | Recombinant vaccinia viruses for the characterization of Plasmodium falciparum-specific cytotoxic T lymphocytes: recognition of processed antigen despite limited re-stimulation efficacy. |
| title_full | Recombinant vaccinia viruses for the characterization of Plasmodium falciparum-specific cytotoxic T lymphocytes: recognition of processed antigen despite limited re-stimulation efficacy. |
| title_fullStr | Recombinant vaccinia viruses for the characterization of Plasmodium falciparum-specific cytotoxic T lymphocytes: recognition of processed antigen despite limited re-stimulation efficacy. |
| title_full_unstemmed | Recombinant vaccinia viruses for the characterization of Plasmodium falciparum-specific cytotoxic T lymphocytes: recognition of processed antigen despite limited re-stimulation efficacy. |
| title_short | Recombinant vaccinia viruses for the characterization of Plasmodium falciparum-specific cytotoxic T lymphocytes: recognition of processed antigen despite limited re-stimulation efficacy. |
| title_sort | recombinant vaccinia viruses for the characterization of plasmodium falciparum specific cytotoxic t lymphocytes recognition of processed antigen despite limited re stimulation efficacy |
| work_keys_str_mv | AT aidoom recombinantvacciniavirusesforthecharacterizationofplasmodiumfalciparumspecificcytotoxictlymphocytesrecognitionofprocessedantigendespitelimitedrestimulationefficacy AT lalvania recombinantvacciniavirusesforthecharacterizationofplasmodiumfalciparumspecificcytotoxictlymphocytesrecognitionofprocessedantigendespitelimitedrestimulationefficacy AT whittleh recombinantvacciniavirusesforthecharacterizationofplasmodiumfalciparumspecificcytotoxictlymphocytesrecognitionofprocessedantigendespitelimitedrestimulationefficacy AT hilla recombinantvacciniavirusesforthecharacterizationofplasmodiumfalciparumspecificcytotoxictlymphocytesrecognitionofprocessedantigendespitelimitedrestimulationefficacy AT robsonk recombinantvacciniavirusesforthecharacterizationofplasmodiumfalciparumspecificcytotoxictlymphocytesrecognitionofprocessedantigendespitelimitedrestimulationefficacy |